EFEK CHLORHEXIDINE DIGLUCONATE 2% SEBAGAI CAVITY CLEANSER TERHADAP INFILTRASI SEL INFLAMASI PADA PULPA GIGI TIKUS SPRAGUE DAWLEY

• Main Authors: , ARIFAH HARIADI, , drg. Tetiana Haniastuti, M.Kes., Ph.D
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123927/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=64044
• Institution: Universitas Gadjah Mada

Chlorhexidine digluconate 2% is a cavity cleanser, which widely used in the practice of dentistry. Although this agent has a broad spectrum of antibacterial effect, it has a sitotoxic effect to odontoblast-like cells as well. Therefore, it may induced inflammation. Inflammation itself means a respond of our body to extinguish any foreign material which already entering the tissue or damaged tissue due to irritations. Pholymorfonuclear cell (PMN) and macrophage are inflammation cells which play a major role of inflammation by phagocyting foreign materials. The objective of this research was to study the effect of chlorhexidine digluconate 2% as a cavity cleanser on the inflammatory cells infiltration on the pulp of Sprague Dawley rats. Sprague Dawley rats were divided into treatment and control groups. A cavity was made with 0,9mm round diamond bur on the occlusal area of the rats� left and right upper first molar. Chlorhexidine digluconate 2% was applied on the cavities of the treatment group while aquadest was applied on the control group�s cavities for fifteen seconds. The rats were sacrificed at 1 hour, 3, 5, and 7 days after treatment. The maxillary tissue of the rats were then processed for histological preparations and stained with Hematoxilin Eosin. PMNs and macrophages were examined using a light microscope with 400x magnification. The data was analyzed using ANOVA and LSD (p<0,05). The results of ANOVA showed that there were significant differences between the treatment and control groups on every exposure times. In conclusion, the application of chlorhexidine digluconate 2% as cavity cleanser induced the inflammatory cells infiltration on the pulp of Sprague Dawyley rats. The inflammatory cells reached the maximum amount 3 days after exposure.