DEGRADASI C.I. REACTIVE ORANGE 86 MENGGUNAKAN Pseudomonas aeruginosa FNCC-0063
A Pseudomonas aeruginosa strain expressing azoreductase activity was utilized to degrade an azo dye Reactive Orange 86 (RO 86). The effect of various parameters such as agitation process, incubation time, the number of bacterial colonies, pH, and initial concentration of solution were investigated....
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/125398/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=65566 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | A Pseudomonas aeruginosa strain expressing azoreductase activity was
utilized to degrade an azo dye Reactive Orange 86 (RO 86). The effect of various
parameters such as agitation process, incubation time, the number of bacterial
colonies, pH, and initial concentration of solution were investigated. The product
resulting from bacterial degradation was analyzed with spectrophotometer UVvisible
and compared with the initial spectra of dye solution.
The results show that Pseudomonas aeruginosa can not decolorize RO 86
under agitation condition. The optimum degradation of RO 86 occurred after 96
hours incubation time and pH 6 under static incubation. The increase in the
number of bacterial colonies enhanced the efficiency of degradation into optimum
condition at 2,3 x 10
10
cfu of bacterial colonies, while the increase in the initial
dye concentration lowered the efficiency of dye degradation. The UV-visible
analysis of the compunds resulting from bacterial degradation suggests that the
molecular structure of RO 86 changes after degradation and it can be inferred that
the azo bond is cleaved during the degradation, but the dye is not completely
degraded to be simpler molecules. |
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