IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE
This research aims to identify the compounds of the active fractions from the brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The study includes sampling, identification of the seaweed, extraction, separation by column chromatography, identification by Liquid Chromatography...
Saved in:
Main Authors: | , |
---|---|
Format: | Theses and Dissertations NonPeerReviewed |
Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
|
Subjects: | |
Online Access: | https://repository.ugm.ac.id/125858/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66039 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Gadjah Mada |
id |
id-ugm-repo.125858 |
---|---|
record_format |
dspace |
spelling |
id-ugm-repo.1258582016-03-04T08:39:50Z https://repository.ugm.ac.id/125858/ IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. ETD This research aims to identify the compounds of the active fractions from the brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The study includes sampling, identification of the seaweed, extraction, separation by column chromatography, identification by Liquid Chromatography-Electrospray Ionization Mass Spectrometry (LC-ESIMS) and activity test. The weight of fractions I, II and III are 0.03%, 0.05%, and 0.03%, respectively. Identification by ESI-MS fractions I and II showed the precent of loliolide. Fraction I also contained halogenated furanone, whereas fraction III 4,4�-dihydroxybiphenyl compound. The activity test of each fractions carried out in vitro using -glucosidase enzyme and the substrate of p-nitrophenyl- -D-glucopiranoside. The product of reaction was measured its absorbance using the Elisa microplate reader at 405 nm. At the concentration of 40 μg/mL, fraksi I, II and III inhibits the -glucosidase enzyme activity by 89.8%, 95.0%, and 96.1%, respectively. IC50 values of fractions I, II and III, were 8.9, 6.3, and 7.6 μg/mL, respectively. While IC50 value of an antidiabetic drugs of akarbose was only 2.1 mg/mL. Statistical tests-one way ANOVA with 95% confidence level was carried out to distinguish between the IC50 values of each fraction and fraction against of acarbose, with the results differ significantly (P < 0.05). [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , LIS FITRAMADAN and , Respati Tri Swasono, S.Si., M.Phil., Ph.D. (2013) IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66039 |
institution |
Universitas Gadjah Mada |
building |
UGM Library |
country |
Indonesia |
collection |
Repository Civitas UGM |
topic |
ETD |
spellingShingle |
ETD , LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI INHIBITOR ENZIM -GLUKOSIDASE |
description |
This research aims to identify the compounds of the active fractions from the
brown seaweed Sargassum hystrix extract as -glucosidase enzyme inhibitor. The
study includes sampling, identification of the seaweed, extraction, separation by
column chromatography, identification by Liquid Chromatography-Electrospray
Ionization Mass Spectrometry (LC-ESIMS) and activity test. The weight of
fractions I, II and III are 0.03%, 0.05%, and 0.03%, respectively. Identification by
ESI-MS fractions I and II showed the precent of loliolide. Fraction I also
contained halogenated furanone, whereas fraction III 4,4�-dihydroxybiphenyl
compound. The activity test of each fractions carried out in vitro using
-glucosidase enzyme and the substrate of p-nitrophenyl- -D-glucopiranoside.
The product of reaction was measured its absorbance using the Elisa microplate
reader at 405 nm. At the concentration of 40 μg/mL, fraksi I, II and III inhibits the
-glucosidase enzyme activity by 89.8%, 95.0%, and 96.1%, respectively. IC50
values of fractions I, II and III, were 8.9, 6.3, and 7.6 μg/mL, respectively. While
IC50 value of an antidiabetic drugs of akarbose was only 2.1 mg/mL. Statistical
tests-one way ANOVA with 95% confidence level was carried out to distinguish
between the IC50 values of each fraction and fraction against of acarbose, with the
results differ significantly (P < 0.05). |
format |
Theses and Dissertations NonPeerReviewed |
author |
, LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. |
author_facet |
, LIS FITRAMADAN , Respati Tri Swasono, S.Si., M.Phil., Ph.D. |
author_sort |
, LIS FITRAMADAN |
title |
IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_short |
IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_full |
IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_fullStr |
IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_full_unstemmed |
IDENTIFIKASI SENYAWA DALAM FRAKSI AKTIF EKSTRAK
RUMPUT LAUT COKLAT Sargassum hystrix SEBAGAI
INHIBITOR ENZIM -GLUKOSIDASE |
title_sort |
identifikasi senyawa dalam fraksi aktif ekstrak
rumput laut coklat sargassum hystrix sebagai
inhibitor enzim -glukosidase |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2013 |
url |
https://repository.ugm.ac.id/125858/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66039 |
_version_ |
1681232334819426304 |