EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA)

• Main Authors: , TITIK NURYASTUTI, , dr. Praseno, SpMK(K)
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/126194/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66396
• Institution: Universitas Gadjah Mada

Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by these bacteria are often very severe and difficult to treat because of the limited sensitivity of antibiotics and the development of antibiotic resistance is very fast. Biofilm formation is an important factor in the pathogenicity of Staphylococcus infections, since the bacteria can attach to and colonizes in biomaterials devices. Biofilm is one of the reason of antibiotic therapy failure. In this study, the ability of MRSA isolate (Methicillin Resistant Staphylococcus aureus) and MSSA (Methicillin Sensitive Staphylococcus aureus) to form biofilm were studied by congo red agar and microtiter plate test. PCR was performed to detect the presence of icaA/D, which responsible for biofilm production. Antibiotic sensitivity was done against MSSA and MRSA isolates by agar diffusion method. The results of this study showed that 90 % of MRSA isolates were not able to form biofilm, whereas all MSSA isolates have ability to form biofilm, although all MRSA and MSSA isolates harbour both genes icaA/D in the chromosomes. MRSA isolates were more resistant to many classes of antibiotics than MSSA. Antibiotics still potent for most of the MRSA isolates were chloramphenicol, erythromycin and cotrimoxazole. There seems to be differences in the mechanisms of regulation and biofilm formation in MRSA and MSSA isolates. The presence of SSC mec in MRSA bacterial chromosome, will repress gene controlling of biofilm formation, so MRSA prefers to express the antibiotic resistance genes which can be used to adapt in clinical situation.