VARIASI GENETIK POPULASI LOBSTER HIJAU PASIR (Panulirus homarus L.) DI LOMBOK BERDASARKAN PENANDA MOLEKULAR
Panulirus homarus is one of the high economically valued spiny lobster which is mostly caught and cultured at south coast of Lombok Island among the other spiny lobsters. The purposes of this reasearch are to observe the genetic variations within Panulirus homarus populations in Lombok by Inter-Simp...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/126239/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66446 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Panulirus homarus is one of the high economically valued spiny lobster
which is mostly caught and cultured at south coast of Lombok Island among the
other spiny lobsters. The purposes of this reasearch are to observe the genetic
variations within Panulirus homarus populations in Lombok by Inter-Simple
Sequence Repeats (ISSR) markers, and to determine which population in Lombok
is the most potential for juvenile harvesting, parents selection, dan conservation.
Sampling was done in three locations, which are Gerupuk Bay, Bumbang Bay and
Awang Bay. DNA amplification was done by PCR-ISSR method using ISSR1 and
ISSR3 primers. Data analysis was done by UPGMA method to construct a
dendrogram. Morphological characters of body width/length ratio and coloration
do not show significant differences between the three populations. There were
total of 48 polymorphic loci amplified from the samples of three analyzed
populations. Population in Gerupuk Bay has the highest genetic variations in the
ISSR regions among the three analyzed populations based on their polymorphism
percentages and similarity values. Branching pattern of the dendrogram shows the
clustering of 23 analyzed samples into three distinct groups consistent with the
three sampling locations. |
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