AKTIVITAS ANTIOKSIDAN EKSTRAK KLOROFORM DAN METANOL DAUN Piper spp.
Piper is one of the largest genera of flowering plants. Traditionally, this plant is used for medicinal purposes.This study aims to determine the relationship between the content of the antioxidant compound with the highest antioxidant activity in Piper leaves methanol and chloroform extracts. Piper...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/126283/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66499 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Gadjah Mada |
| Summary: | Piper is one of the largest genera of flowering plants. Traditionally, this
plant is used for medicinal purposes.This study aims to determine the relationship
between the content of the antioxidant compound with the highest antioxidant
activity in Piper leaves methanol and chloroform extracts. Piper leaves were dried
and macerated using chloroform and methanol. Obtained extract was tested by
DPPH antioxidant activity, total phenolic content and total flavonoid content.
Species with highest antioxidant activity was tested the ability of the extract to
inhibit the oxidation by hydroxyl radicals on DNA. Antioxidant activity was
analyzed using ANOVA. The results show that the highest antioxidant activity
was P. acutilimbum leaves methanol extract with inhibition values 70.81 %,and
quersetin by 94,17 %. The highest total phenol content was the P. acutilimbum
leaves methanol extract 39.46 mgGAE/g. The highest total flavonoid content was
P. majusculum leaf chloroform extract 46.62 mgQE/g. Good relationships
between phenolic content and radical DPPH scavenging activity (R= 0,6882) was
found. Comparison of antioxidant activity between P. acutilimbum stems and
leaves obtained that the leaves have higher antioxidant activity than the stem with
each value is 70.81 % and 9.24 %. Methanol extract of P. acutilimbum leaves with
concentrations of 150 μg/mL and 150 μg/mL of quersetin were able to inhibit the
oxidation reaction caused by hydroxyl radicals on the plasmid. From these results,
it can be concluded that Piper leaves have a potential source as the antioxidant
compounds, especially P. acutilimbum leaves. |
|---|