KARAKTERISTIK FISIKOKIMIA DAN POTENSI PREBIOTIK EKSTRAK DAN HIDROLISAT ENZIMATIK LAMINARAN Sargassum crassifolium
One species of brown algae potential producer of alginate is Sargassum. In the making of alginate, water-soluble polysaccharides such as fucoidan and laminaran are dissipated as waste. Fucoidan has been used but laminaran has not been in efficient utilization, so to conduct a research is essential t...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Online Access: | https://repository.ugm.ac.id/126400/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66620 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | One species of brown algae potential producer of alginate is Sargassum. In the
making of alginate, water-soluble polysaccharides such as fucoidan and laminaran
are dissipated as waste. Fucoidan has been used but laminaran has not been in
efficient utilization, so to conduct a research is essential to find its potential.
The general objective of this study was to obtain laminaran prebiotic of brown algae
S.crassifolium. The specific objectives of this study were (1) to obtain an efficient
laminaran extraction method and physico-chemical, chemical and functional
characteristics of laminaran crude extract (2) to obtain the enzymatic hydrolysis or
depolymerization time of laminaran crude extract producing laminaran
oligosaccharides with DP less than equal 9 (bifidogenic), (3) to determine the effect
of treatment process and gastric juice to chemical stability of laminaran prebiotic
oligosaccharides, (4) to determine the index of laminaran prebiotic oligosaccharides
compared with standard laminaran and commercial prebiotics (inulin) and (5) to
determine the ability of laminaran oligosaccharides in producing SCFA on
fermentation in vitro using bacteria from feces.
The best extraction method was the method 5 (Yvin modification) that algae powder
was extracted with acid filtrate asulfat then precipitated with ethanol followed by reextraction
of algae residue with distilled water and the resulting filtrate was also
precipitated with ethanol. The extraction of brown algae S. crassifolium using Yvin
modification method produced two types of extracts i.e. is laminaran extract lysis
with acid so that the result still contained many impurities from the cell wall
components its names LAE (Laminaran Acid Extraction) and laminaran extracts
resulted from the washing of algae residue with distilled water so that its main
content was LME (Laminaran Modified Extraction).
The physico-chemical characterization and chemical such as WHC and ash content
LAE(11,54 mg/g |
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