KEMAMPUAN REGENERASI KALUS EMPAT KLON TEBU (Saccharum officinarum L.)

• Main Authors: , RIZA LUTHFIAH, , Dr. Ir. Taryono, M.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127333/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67583
• Institution: Universitas Gadjah Mada

Sugarcane is one of the important agricultural commodity that has high economic value as raw material for sugar industry. One of the obstacle is large scale seed availability due to the limitation of planting material�s area. Plant tissue culture is an alternative to overcome the problems. Callus culture and shoot regeneration are a contributing factor in plant tissue culture, but there is a problem in the long-term maintenance of callus which is the decreasing callus regeneration potential. This research used two stages, (1) callus induction with single factor experiment arranged in Completely Randomized Design, and (2) shoot regeneration using 2 factors experiment in Completely Randomized Design and 5 replications. The first factor is sugarcane clones (PS 862, PS 864, PS 881, and VMC 86-550), and the second factor is subculture frequencies which is transferred in every three weeks i.e. third, sixth, ninth, and twelfth week. The explant source used is an immature leaves roll sugarcane (3 � 4 month). Callus induction and subculture used MS medium + 1.5 mg/l 2,4-D, whereas shoot regeneration used MS medium + 2.0 mg/l IAA + 2.0 mg/l IBA + 2.0 mg/l kinetin. Observed variables includes callus emerge, shoots regeneration rate and number of shoot. Statistical analysis using ANOVA showed significant difference in the rate of callus induction. PS 862 clones has the fastest callus emerge, shoots growth, and the largest number of shoots. Interaction between clones and subculture frequency occurs in time and number of shoots.