EKSPRESI TEMPORAL GEN Ehd1 PADA PADI HITAM (Oryza sativa L. �CEMPO IRENG�)

• Main Authors: , ANISA NAZERA FAUZIA, , Dr. Yekti Asih Purwestri, M.Si.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127491/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67747
• Institution: Universitas Gadjah Mada

Black rice (Oryza sativa L.) cultivars Cempo Ireng is one of the original local rice in Yogyakarta. This rice contains anthocyanin and iron but has a long harvest period. The harvest is closely related with flowering time. Ehd1 is one of the genes involved in the induction of flowering. The gene promotes flowering through the upstream gene of Hd3a dan RFT1. Hd3a and RFT1 are the key gene of flowering genes. The expression of Ehd1 gene is an early indicator of flowering. The objective of this research was to determine the temporal expression of Ehd1 in Cempo Ireng rice cultivar. The first method was planting Cempo Ireng rice cultivar in greenhouse Faculty of Agriculture with cultivating media consist of paddy soil and manure (3:1). Then, RNA was isolated from leave blades at 80, 91, 100, 109, and 120 DAS (Days After Sowing) using TRIzol reagent and RNeasy Plant Mini Kit. RNA isolation results was evaluated qualitatively and quantitatively. One µg of RNA was used for cDNA synthesis using oligo-DT primers, ubiquitin and OsGF14 amplification was performed as internal control, and Ehd1 amplified using specific primers. The results showed that Cempo Ireng rice cultivar entering reproductive stage (heading date) at 118 DAS. The results of RNA isolation qualitatively and quantitatively showed that the yield of RNA isolation using TRIzol has a low ratio but high concentrations. While isolation with RNeasy Plant Mini Kit produced RNA with high ratio but low concentrations. The result of OsGF14 and ubiquitin amplification showed that both genes can be amplified in all samples at various DAS. Ehd1 genes was expressed at 100 and 109 DAS. Ehd1 expression occured before flowering (heading).