HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN STATUS LIMFONODI PADA KARSINOMA PAYUDARA

• Main Authors: , ERYNA AYU NUGRA DESITA, , dr. Irianiwati, Sp.PA(K).
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127742/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68016
• Institution: Universitas Gadjah Mada

Background: Metastasis of breast carcinoma, which occurs primarily through the lymphatic pathway, begins with tumor invasion into lymphatic vessels, then toward lymph nodes, and subsequently to distant organs. Lymph node metastasis is a strong prognostic factor in breast cancer. Tumor associated macrophages (TAMs) are the main inflammatory cells in carcinoma. In breast carcinoma, the protumoral characters of TAMs is more dominant than the antitumoral ones. The role of TAMs in lymph node metastasis in breast carcinoma remains unclear. Objective: To study the relationship between amount of TAMs and lymph node status in breast carcinoma. Method: 46 paraffin blocks of invasive ductal carcinoma of the breast from Sardjito Hospital Yogyakarta were observed. Sampels were stained immunohistochemically by CD68 monoclonal antibody. CD68 expression was assessed from amount of TAMs whose cytoplasm expressed CD68 within 5 hot spot areas. TAMs was grouped into high and low, based on median value as cut-off. Lymph node status was grouped into positive and negative. The relationship between TAMs and lymph node status was analyzed by Chi-Square test. Result: TAMs cut-off point was 48,5. Frequency of high TAMs: 50%, low TAMs: 50%. Frequency of samples with positive lymph node: 65% and negative lymph node: 35%. There was no significant relationship between amount of TAMs and lymph node status (p=0,063). Nevertheless, many samples with high amount of TAMs were found in carcinoma with lymph node metastasis. Conclusion: In this study, the amount of TAMs had no relationship with lymph node status in breast carcinoma.