UJI POTENSI ANTITUBERKULOSIS EKSTRAK ETANOL UMBI BAWANG MERAH (Allium ascalonicum L) TERHADAP MULTIDRUGS RESISTANT Mycobacterium tuberculosis SECARA IN VITRO

• Main Authors: , DYAH FIHARJATIN, , dr.Tri Wibawa, Ph. D
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127812/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68102
• Institution: Universitas Gadjah Mada

Background :Tuberculosis (TB) is an infectious disease caused by Mycobacteriium tuberculosis. In 2011, there were an estimated 1.4 million deaths among 9 million incident of TB cases globally. Nowadays, new problem arises i.e. patients who resistant to at least two first line antituberculosis drugs which is called Multidrugs Resistant Mycobacterium tuberculosis (MDR-TB). Indonesia is one of 27 high MDR-TB burden countries. Treatment of MDR-TB is difficult, expensive, and it has many side effects. Indonesia has various plants available to be developed as herbal medicine such as shallot (Allium ascalonicum L.) The current research evidence showed that shallot extract has antituberculosis activity. Objective : To determine antituberculosis activity and minimum bactericidal concentration (MBC) of ethanolic extract of shallot bulbs in MDR-TB. Methods :The ethanolic extract of shallot bulbs is contacted to MDR-TB cultured in MB 7H9-OADC medium during 10 days. Then, it was inoculated in LJ medium up to 6 weeks to identify the MBC. Result : Positive control grown in first week.PGA was used as emulsifier on the ethanolic extract, however PGA 2% as a control showed a bactericidal activity. The ethanolic extract of shallot bulbs potentially put MDR- TB out at concentration 25%, 12.5%, 6.25%, 3.125%,and 0.78125%. This antituberculosis activity of ethanolic extract of shallot bulbs was caused by allicin that suppressed the expression of antigen 85B by inhibit gluthation peroxidase in MDR-TB, and also quercetin that inhibit MDR-TB growth by targeted DNA gyrase subunit B. Conclusion : Extract ethanolic of the shallot bulbs suspended in PGA 2% potentially put MDR-TB out. The MBC was less than 0.78125%. Keywords : Allium ascalonicum L, Multidrugs Resistant Mycobacterium tuberculosis, antituberculosis.