PENGARUH PEMBERIAN ANTIBIOTIK PIPERASILIN, GENTAMISIN, DAN CAMPURAN (PIPERASILIN DAN GENTAMISIN) TERHADAP PERTUMBUHAN Proteus spesies PADA KULTUR Mycobacterium tuberculosis DENGAN MEDIUM CAIR MGIT

• Main Authors: , ZULFA FAIQOH, , Dra. Ning Rintiswati, M.Kes.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/127955/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68276
• Institution: Universitas Gadjah Mada

Tuberculosis (TB) is a disease caused by the bacterium Mycobacterium tuberculosis. It is the second killer among infectious diseases after Human Immunodeficiency Virus (HIV). Use of MGIT (Mycobacterium Growth Indicator Tube) as a culture medium in the diagnosis of tuberculosis requires a relatively shorter time compared to solid medium. However, as a liquid medium, many bacterial contaminants can grow in this medium, included Proteus sp. Anticipation for the presence of these contaminants need to be done with the use of broad-spectrum antibiotics, such as Piperacillin and Gentamicin. The purpose of this study was to determine the potential of Piperacillin, Gentamicin, or a mixture (Piperacillin and Gentamicin) as an anti-contaminant to Proteus spp. in culturing Mycobacterium tuberculosis by MGIT liquid medium. This study used cultures of Mycobacterium tuberculosis and Proteus sp. (104 μg/ml) are available in the Laboratory of Microbiology, Faculty of Medicine, Gadjah Mada University. The cultures were performed in a MGIT medium which is divided into a control group, they are Mycobacterium tuberculosis control and Proteus spp. control, as well as the treatment groups, they are Piperacillin group, Gentamicin group, and mixed group with MIC value was 2 μg/ml for Piperacillin and 8 μg/ml for Gentamicin. Further, observe the growth of bacteria. The results of this study was that there is growing of Mycobacterium tuberculosis and Proteus spp. in Piperacillin group indicated by the presence of Mycobacterium tuberculosis specific turbidity and AFB on ZN�s staining for Mycobacterium tuberculosis, as well as the growth of colonies on MacConkey culture and gram-negative bacteria on Gram's staining for Proteus spp. While in the Gentamicin group and mixture group there were negative of both bacterial growth. The conclusion of this study is the Piperacillin and Gentamicin antibiotic is less potential as an anti-contaminant to Proteus spp. in the culturing of Mycobacterium tuberculosis by MGIT liquid medium. Further research needs to be done using broad-spectrum antibiotics in addition to Piperacillin and Gentamicin.