LEPTIN GENE POLYMORPHISM IN ISCHEMIC STROKE PATIENTS WITH INSULIN RESISTANCE GRADUATING PAPER
BACKGROUND: Ischemic stroke is a heterogeneous disease with various underlying pathophysiology. Genetic factor plays a crucial role among non-modifiable risk factors. OBJECTIVE: This research aims to identify leptin gene polymorphism as a risk factor for insulin resistance and ischemic stroke. METHO...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/128113/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68444 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | BACKGROUND: Ischemic stroke is a heterogeneous disease
with various underlying pathophysiology. Genetic factor
plays a crucial role among non-modifiable risk factors.
OBJECTIVE: This research aims to identify leptin gene
polymorphism as a risk factor for insulin resistance
and ischemic stroke.
METHOD: This research consists of a total of 84
samples, 42 cases and 42 controls. Case refers to blood
sample of ischemic stroke patient while control refers
to blood samples of non-ischemic stroke patient with no
history of stroke. Blood samples of all subjects
underwent DNA isolation and Polymerase Chain Reaction
(PCR) and electrophoresis were done and visualized by
staining with ethidium bromide and viewed under
ultraviolet (UV) rays to study the polymorphism of
microsatellite allele of Leptin gene in these samples.
RESULTS: Results showed that there is no direct
correlation between leptin gene polymorphism, incidence
of ischemic stroke and insulin resistance. Correlation
of leptin genotype with ischemic stroke (OR=0.459 |
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