LARVICIDAL EFFECT OF CHLOROFORM EXTRACT OF PANDAN LEAVES (Pandanus amaryllifolius Roxb) AGAINST Aedes aegypti LARVAE

• Main Authors: , ANNA ASMAUL MARDIYAH, , dr. Tri Baskoro Tunggul Satoto, M.Sc, Ph.D
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128124/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68455
• Institution: Universitas Gadjah Mada

Dengue Fever (DF) and Dengue Haemorrhagic Fever DHF), is one of the major health problem afflicting Indonesia. Dengue Fever (DF) is caused by an arthropodbourne virus of the genus Flavavirus, within the family Flavaviridae, which is transmitted through the bite of female Aedes aegypti mosquitoe. Elimination of vector can be done by environmental management, biological control, chemical control (WHO, 2004). Various research and experiment done shows that Pandanus amaryllifolius Roxb. leaves contain several compounds that can be used as natural insecticide. Previous study has demonstratedIt is containing poliphenol, flavanoid, saponin, volatile oil, and alkaloid. Aim of study: To determine whether there is larvicidal effect of chloroform extract of pandan leaves ( P. Amaryllifolius) againts Ae. aegypti larvae and the effective concentration of LC50 and LC90 of the extract, and also to establish whether an increase in concentration of thr extract cause an increase in the mortality of larvae. Method: The test is performed by introducing a set amount of chloroform extract of P. amaryllifolius leaves against Ae. aegypti larvae of the 3 rd and 4 th instar, with variation of concentration, under standarized bioassay methods. Result: The LC50 and LC90 of chloroform extract of P. amaryllifolius leaves against Ae. aegypti larvae is 221.67 ppm and 855.58 ppm, respectively. Conclusion: There is larvicidal effect of chloroform extract of pandan leaves ( P. amaryllifolius Roxb.) againts Ae. aegypti larvae and with LC50 is 221.67 ppm and LC90 855.58 ppm. An increase in concentration of the extract cause an increase in the mortality of larvae.