PENGARUH PEMBERIAN ANTIBIOTIK PIPERASILIN, GENTAMISIN DAN CAMPURANNYA TERHADAP PERTUMBUHAN Staphylococcus aureus PADA KULTUR Mycobacterium tuberculosis DENGAN MEDIUM CAIR MGIT

• Main Authors: , CITA NUANSA IMANE PUTRI I, , Dr. Dra. Ning Rintiswati, M.Kes
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128356/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68696
• Institution: Universitas Gadjah Mada

Background : Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis. Tuberculosis is one of the unslovable global health problem. The gold standard to diagnose tuberculosis is bacterial culture. MGIT (Mycobacterium Growth Indicator Tube)is one of liquid medium tuberculosis that requires a relatively shorter time compared to culture in solid medium. However, MGIT has a weakness where the contaminants are easily to growth. One of the bacteria that is often as a contaminant is Staphylococcus aureus. Anticipation for the presence of these contaminant need to be done by using antibiotics such as Piperacillin and Gentamicin. Objective : To determine the effect of antibiotic Piperacillin, Gentamicin or the mixture against Staphylococcus aureusin culture of Mycobacterium tuberculosis by MGIT liquid medium. Methods: This study used a culture of Mycobacterium tuberculosis and Staphylococcus aureus (104 μg/ml)that are available in Laboratory of Microbiology, Faculty of Medicine, University of Gadjah Mada. The culture that�s performed in MGIT, is divided into control group,which consist of control Mycobacterium tuberculosis and control Staphylococcus aureus, as well as the treatment groups, ie groups of Piperacillin, Gentamicin groups, and mixed groups with MIC 2 μg/ml for Piperacillin and 8 μg/ml for Gentamicin. Then, there�s further observation on the growth of bacteria. Results: There was a growth of Mycobacterium tuberculosis in Piperasillin group without Staphylococcus aureus growth, that�s demonstrated by the Mycobacterium tuberculosis-specific turbidity and visually BTA on staining ZN, while there were no growth any bacteria in gentamicin group and mixture group. Conclusion: Piperacillin can be used to reduce the growth of Staphylococcus aureus in MGIT. However, the antibiotics gentamicin and Mixed (Piperacillin and Gentamicin) was unable to inhibit the growth of Staphylococcus aureus in MGIT.