EFEK EKSTRAK TERPURIFIKASI Andrographis paniculata (Burm. f.) Nees DAN ANDROGRAFOLID TERHADAP INDEKS HOMA-IR TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR DENGAN DIET TINGGI LEMAK DAN FRUKTOSA

• Main Authors: , IGNATIUS RYAN ADRIAWAN, , dr. Rina Susilowati, Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128414/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68759
• Institution: Universitas Gadjah Mada

Background: Early stage of type 2 diabetes mellitus pathogenesis includes insulin resistance, shown by increment of homeostasis model assessment � insulin resistance (HOMA-IR) index. A. paniculata is known to have antihyperglycemic effect, but its effect on HOMA-IR index of high fat and fructose fed Wistar rats is unknown. Objectives: To see whether A. paniculata purified extract and andrographolide modify HOMA-IR index of high fat and fructose fed Wistar rats, and determine form and dosage that yields the best result. Method: Thirty eight Wistar rats were divided into 7 groups, ND (given standard chow diet for 55 days), HFFD (given combination of 80% standard chow, 15% lard, 5% duck egg yolk, and fructose 180 mg/100 gBB for 55 days), HFFD-Met (HFFD for 55 days, metformin 45 mg/kgBB for the last 5 days), HFFD-E1 and E2 (HFFD for 55 days, A. paniculata purified extract 434,6 mg/kgBB and 1.303,8 mg/kgBB for the last 5 days), HFFD-A1 and A2 (HFFD for 55 days, andrographolide 1,5 mg/kgBB and 4,5 mg/kgBB for the last 5 days). Sera were collected on the 55th day to measure the fasting glucose and insulin concentrations, and to calculate HOMA-IR index. The three data were analysed using Kruskal-Wallis and Mann-Whitney tests. Results: Mean fasting insulin concentration of HFFD-E1 and E2 (0,40 ng/ml) is lower than those given andrographolide. Mean fasting glucose concentration of HFFD-E2 (73,40 mg/dl) is lower than other treatment groups, even HFFD-Met (74,33 mg/dl) which is the positive control group. Mean HOMA-IR index of HFFD-E2 (0,07) is lower than other treatment groups except HFFD-Met (0,06). Conclusion: Both A. paniculata purified extract and andrographolide reduces HOMA-IR index of high fat and fructose fed Wistar rats. The greatest HOMA-IR index reduction is yielded by A. paniculata purified extract with the dosage of 1.303,8 mg/kgBB.