EFFECT OF METHANOL EXTRACT OF PANDAN LEAVES (Pandanus Amaryllifolius Roxb) AGAINST Culex Quinquefasciatus AS LARVICIDE
Background: Bancroftian filariasis is among the diseases transmitted by mosquito and Culex quinquefasciatus is the main vector in South East Asia. Filariasis elimination programme of Indonesia includes control of vector. One of the means is by larviciding. Uncontrolled use of larvicide results in la...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/128453/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68798 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Background: Bancroftian filariasis is among the
diseases transmitted by mosquito and Culex
quinquefasciatus is the main vector in South East Asia.
Filariasis elimination programme of Indonesia includes
control of vector. One of the means is by larviciding.
Uncontrolled use of larvicide results in larvicide
resistance in Culex species. It poses the need of new
alternative larvicides. Since Cx. quinquefasciatus
breeds in close proximity to humans, the safety of
larvicide becomes crucial. Therefore, Pandanus
amaryllifolius Roxb may be potential alternative
larvicide against Cx. quinquefasciatus larvae as it
contains bioactive chemicals with larvicidal potency.
Objective: To explore biopotency of methanol extract of
P. amaryllifolius R. leaves as larvicide against Cx.
quinquefasciatus larvae. To find out the relation
between variance of extract concentrations and larval
mortality.
Design: This study is true experimental posttest only
study. It started with preliminary test to obtain range
of extract concentrations with 20%-80% larval
mortality. Then, based on th preliminary result, range
of seven extract concentrations for final test was
calculated and three test repeatitions were conducted.
Subjects were 4 to 5-day old Cx. quinquefasciatus
larvae (Instar III or IV). Observation of larval
mortality was done after 24 hours and data was analysed
using probit analysis
Results: LC50 and LC90 values are at 1361.308ppm and
80974.59ppm respectively. Larve response to the
exposure was heterogenous with equation of regression
line Y=0.7223523X + 2.736179.
Conclusion: Methanol extract of Pandanus amaryllifolius
Roxb leaves has larvicidal effect against instar III/IV
larvae of Culex quinquefasciatus but ineffective. There
is linear relationship between extract concentration
and larval mortality. |
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