COMPARING THE SENSITIVITY AND SPECIFICITY OF ZINC SULPHATE FLOTATION METHOD TO FORMOL ETHER SEDIMENTATION IN IDENTIFYING INTESTINAL PROTOZOA

• Main Authors: , DINI ALYANI, , dr. Elsa Herdiana M.Kes, PhD
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128468/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68815
• Institution: Universitas Gadjah Mada

Background: A simple microscopic examination of stool should be carried out for the diagnosis of intestinal protozoa infection. When the negative result is obtained from this direct method, the concentration method should be utilized and often gives a positive result. There are two concentration methods that are routinely performed as diagnostic tools those are Formol-ether sedimentation method and Zinc-sulpahte flotation method. Each method has different procedure and limitations. Some researchers recommend using both method since neither technique alone can identify all parasites in the fecal sample. However this approach is unpractical for most laboratories. This study was done to conclude which method that has higher sensitivity and specificity. Objective: To know the sensitivity and specificity of Formol-ether sedimentation method and Zinc-sulpahte flotation method in identifying intestinal protozoa cyst. Method: Thirty preserved fecal samples were obtained from Parasitology Lab of Faculty of Medicine in Gadjah Mada University. Each sample was tested using both Formol-ether sedimentation (Ritchie�s method) and Zinc-sulphate (Faust�s method) and then microscopically examined to find the intestinal protozoa cysts. Numbers of samples with cystpositive were recorded to determine which method has higher ability to detect the intestinal protozoa cyst. Result: From all the five identified cysts (Entamoeba histolytica, Giardia lamblia, Entamoeba coli, Iodmaeba butschlii, Blastocystis hominis), Ritchie�s method was able to detect more cyst than Faust�s method. The sensitivity and specificity of Ritchie�s method compared to Faust�s are 100% and 93.33% respectively. The Positive Predictive Value (PPV) and Negative Predictive Value (NPV) of Ritchie�s compared to Faust method are 93.75% and 100% respectively. Conclusion: This study concludes that the Ritchie�s method has high sensitivity and specificity compared to Faust�s method in detecting five common intestinal protozoa.