PENGARUH APLIKASI SISTEM PERANCAH NON FREEZE-DRYING CHA-GELATIN SHEET PADA PROLIFERASI DAN EKSPRESI GEN DIFERENSIASI OSTEOGENIK Runx2, Osterix DAN Osteocalcin SEL PUNCA MESENKIM DARI TALI PUSAT BAYI
Bone has a limited ability to perform self-repair and tissue regeneration, thus becoming the world's health problems. Bone tissue engineering is found to be great solution of bone regeneration therapy. The three basic elements in bone tissue engineering are (1) progenitor cells, (2) extracellul...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/128631/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68988 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Bone has a limited ability to perform self-repair and tissue regeneration, thus
becoming the world's health problems. Bone tissue engineering is found to be great
solution of bone regeneration therapy. The three basic elements in bone tissue
engineering are (1) progenitor cells, (2) extracellular matrix scaffold system as
required for the initial proliferation, migration and differentiation of cells, and (3)
growth factors. Carbonate Apatite (CHA)-gelatin sheet, as a scaffold system for bone
tissue engineering, has been developed by Tissue Engineering team in Faculty of
Dentistry, University of Gadjah Mada. The mesenchymal stem cells also known as
progenitor cells that have osteoblast-lineage potential. Therefore, in order to design a
bone implant that can be tolerated human body, it is need to be tested the effect of the
application system based on a synthetic scaffold CHA-gelatin sheet on the ability of
proliferation and osteogenic differentiation of mesenchymal stem cells.
In this study, mesenchymal stem cells obtained from human umbilical cord
because it is one of the richest source of mesenchymal stem cells, and can be isolated
easily and non-invasive by explant method. CHA-gelatin sheet was fabricated with
two variations of the concentration (solid dissolved in liquid), which are 10 % w/v
(CHA/gel-10) and 5% w/v (CHA/gel-05). Each variant uses 25% CHA and 75 %
gelatin ratio. Proliferation test performed using the MTT method to determine the
ability of proliferation induced by CHA-gelatin scaffold sheet on mesenchymal stem
cells. Furthermore, semi-quantitative analysis of gene expression of osteogenic
markers Runx2 , Osterix and Osteocalcin using RT-PCR was performed to determine
the ability of osteogenic differentiation induction system CHA-gelatin scaffold sheet
on mesenchymal stem cells .
The results of this study indicate CHA/gel-05 scaffold capable of inducing
proliferation of mesenchymal stem cells during 7 days of observation time. Then on
osteogenic differentiation observation, scaffolding CHA/gel-10 able to increase the
expression of Osterix and Osteocalcin genes of mesenchymal stem cells on day 10 of
incubation time. Thus CHA-gelatin sheet scaffold are known to have the ability to
induce proliferation and increase osteogenic differentiation of mesenchymal stem
cells from human umbilical cord . However, it still needs further in-vivo testing |
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