POTENSI AKTIVITAS ANTIVIRAL EKSTRAK ETIL ASETAT DAN EKSTRAK AIR METABOLIT SEKUNDER AKTINOMISETES TERHADAP VIRUS DENGUE SEROTIPE-2 (DENV-2)

Dengue virus (DENV) infections are one of the most prevalent infectious diseases in many parts of the world and affecting millions of people annualy. Despite the growing threat from the spread of DENV, there is still no approved antiviral drug for DENV infections. Actinomycetes are well known produc...

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Bibliographic Details
Main Authors: , Dimas Fandi Praditya, , dr. Tri Wibawa, Ph.D.
Format: Theses and Dissertations NonPeerReviewed
Published: [Yogyakarta] : Universitas Gadjah Mada 2014
Subjects:
ETD
Online Access:https://repository.ugm.ac.id/128711/
http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69077
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Institution: Universitas Gadjah Mada
Description
Summary:Dengue virus (DENV) infections are one of the most prevalent infectious diseases in many parts of the world and affecting millions of people annualy. Despite the growing threat from the spread of DENV, there is still no approved antiviral drug for DENV infections. Actinomycetes are well known producers of pharmacological and antiviral agents. Here, we screening ethyl acetate and aqueous extracts from actinomycetes (GMY01, GMY16, GMY29, GMY57, GMR06 and GMR22) for their antiviral activity against dengue virus serotype 2 (DENV-2). BHK-21 cell were infected with DENV-2 and treated with ethyl acetate and aqueous extract. The antiviral activity were analyzed by semi-quantitative RT-PCR and evaluated base on the inhibition of DENV-2 replication. Results revealed all extract from all isolates inhibits in vitro DENV-2 replication in dose dependent maner. The highest inhibition activity was found at the concentration 25 μg/ml for all extract ranging from 80-90 %. These results show the potency of aqueous and ethyl acetate extract from marine actinomycetes as DENV-2 antiviral candidate.