BAKTERI ENDOFIT TANAMAN PURWOCENG (Pimpinella pruatjan Molk.) BERDASARKAN KARAKTER MORFOLOGIS, BIOKIMIAWI DAN MOLEKULAR
Isolation, characterization, identification and antimicrobial screening of endophytic bacteria from purwoceng�s roots (Pimpinella pruatjan Molk.) has been carried out. Isolation of endophytic bacteria was conducted using slices of the root segments. The characterization of endophytic bacteria was...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Online Access: | https://repository.ugm.ac.id/128780/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69147 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Isolation, characterization, identification and antimicrobial screening of
endophytic bacteria from purwoceng�s roots (Pimpinella pruatjan Molk.) has
been carried out. Isolation of endophytic bacteria was conducted using slices of
the root segments. The characterization of endophytic bacteria was conducted
based on morphological, biochemical and molecular characters. Morphological
characters can be seen through the form of colonies, cell shape, edges, elevation
and color of the colony. Biochemical characters were performed by carbohydrate
fermentation, catalase, starch hydrolysis, H2S production, nitrate reduction and
oxidase test. Molecular character based on 16S rRNA gene. Bacterial genomic
DNA obtained was amplified by PCR using specific primers for the 16S rRNA
gene and further characterized by amplified rDNA restriction analysis method
(ARDRA) using restriction enzymes AluI, RsaI, HaeIII and HinfI. Antimicrobial
activity of endophytic bacteria using agar block method. A total of 69 colonies of
endophytic bacteria were isolated and only 30 isolates were successfully purified.
Isolates obtained were dominated by Gram-negative bacteria (70%). The results of
morphological and biochemical characters showed the diversity of endophytic
bacteria isolates. Thrity isolates of endophytic bacteria were identified as
Acidomonas, Amphibacillus, Arsenophonus, Bacillus, Brocothrix, Budvicia,
Cardiobacterium, Cellulomonas, Erwinia, Pantoea, Pediococcus, Plesiomonas
and Proteus. Screening for antimicrobial activity showed only isolate GP11 and
GP12 were potentially inhibit Staphylococcus aureus with the same inhibition
zone is 1,72 cm. Result of fingerprinting DNA by enzymes RsaI digestion
products and HaeIII were able to demonstrate the potential isolates are different
strains. This study suggests that endophytic bacteria can be found in the roots and
have the potential antimicrobial |
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