KECERNAAN IN VITRO MONOGASTRIK BUNGKIL INTI KELAPA SAWIT FERMENTASI MENGGUNAKAN ISOLAT : BAKTERI SELULOLITIK BELALANG KEMBARA (Lokusta migratoria L.)

• Main Authors: , BISMO ADITYO, , Prof. Dr. Ir. Lies Mira Yusiati, SU.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/129385/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69776
• Institution: Universitas Gadjah Mada

This reasearch was conducted to determine the effect of fermentation with the addition of cellulolytic bacteria which had isolated from migratory grasshopper (Lokusta migratoria L) on in vitro digestibility of palm kernel cake (PKC). Cellulolytic bacteria isolated from extract digestive gut of grasshopper that had been enriched and grows. Fermentation PKC was done with different levels of the isolates by 0, 10, 20, and 30% then fermented for 21 days. Fermented PKC was analyzed chemical composition including crude fiber, crude protein, crude fat, dry matter, and organic matter. The digestibility was measured by the method of the monogastric in vitro which including dry matter digestibilty, organic matter digestibility, digested protein content (Lowry) and reducing sugar. Data were analyzed using analysis of variance test pattern in unidirectional the Duncan�s new multiple range test (DMRT). The decline of crude fiber and increased nitrogen free extract on fermented sugar levels rise resulting in a outcome product in vitro. Addition of cellulolytic inoculum levels by 10%, 20% and 30% led to increased levels of reducing sugar content in PKC at 12,39%, 20,33% and 27,98% (P<0,05) compared with controls other than the addition of inokulum by 20% and 30% increased the protein content by 22,25 and 21,74% (P<0,05) compared with controls. Addition of cellulolytic inoculum level by 20 and 30% increased the digestibility of dry matter 11,89 and 13,37% (P<0,05) and organic matter rose by 18,02 and 22,81% (P<0,05) compared to controls. Based on the research that has been done can be concluded that the fermentation of palm kernel cake with the addition of cellulolytic bateria from migratory grasshopper (Lokusta migratoria L) by 20% can increase nutrient digestibility in vitro. Key words : Palm kernel cake fermentation, Cellulolytic isolate, In vitro digestibility, Migratory grasshopper