GAMBARAN HISTOLOGI ORGAN EKSTRAMURAL Iguana iguana SEBAGAI REPTIL HERBIVORA
Iguana iguana is a herbivorous reptile that first domesticated. During last few years, iguana lovers community increase. The problem that often occurs in maintenance of reptiles is digestion. Research on the histological structure of the extramural organ as a support of the digestive system not yet...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/130191/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70606 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Iguana iguana is a herbivorous reptile that first domesticated. During last
few years, iguana lovers community increase. The problem that often occurs in
maintenance of reptiles is digestion. Research on the histological structure of the
extramural organ as a support of the digestive system not yet done. The aims of
this study is to determine the histological structure of extramural organ of Iguana
iguana as herbivorous reptiles.
Three (five month old) Iguana iguana were anastized with chloroform.
Laparatomy was done to collect the extramural organs (liver, vesica fellea,
pancreas, and mandible). Organs were prepared and fixed with Bouin's solution
for 24 hours, transferred to 70% alcohol, and then processed by paraffin method.
Prior to processing, the mandible decalcified using 10% EDTA solution. Tissue
were cut with a thickness of 5μm and continued stained with Hematoxylin Eosin
(liver, vesica fellea, pancreas, mandibular), Alcian Blue-Periodic Acid Schiff
(mandible), and Gomori (mandible).
Histological structure of Iguana iguana�s extramural organs is not different
from other reptiles and mammals in general. Infralabial gland is consist of serous
and mucous cells. Alcian Blue-Periodic Acid Schiff and Gomori staining can
indicate the presence of acid mucopolysaccharides and lipoproteinlipase in
infralabial gland. Liver parenchymal hepatocytes are arranged in the form of
plates radier in the area near the central vein, the lobulation is unclear, hepatic
triad and Melano-Macrophage centers scattered around the central vein. Vesica
fellea is lined by simple columnar epithelium intermitted by pseudostratified
columnar epithelium. Pars exocrine pancreatic gland acinus composed of coneshaped
cells/pyramidal similar to serous cells in salivary gland and duct system is
lined by squamous epithelium, cuboidal epithelium and continue columnar simple
epithelium prior to the duodenum. |
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