ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER DAUN TENDANI (Goniothalamus macrophyllus Hook.f. & Thoms)

• Main Authors: , AGUNG RAHMADANI, , Prof. Drs. Sabirin Matsjeh, Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/130473/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70895
• Institution: Universitas Gadjah Mada

Isolation and evaluation of antibacterial activities of secondary metabolites of tendani leaves (Goniothalamus macrophyllus Hook.f. & Thoms) have been conducted. Tendani leaves were extracted with methanol and fractionated sequentially with n-hexane and ethyl acetate. The ethyl acetate extracts were analyzed using GC-MS and separated by column chromatography to afford 7 fractions. Fractions 4 were rechromatographed to give fraction 4.3. The structure of isolated compound was identified by Mass Spectroscopy and NMR. The GC-MS analysis shows ethyl acetate extracts of tendani leaves contain benzyldehyde compounds, sphatulenol, methyl hexadecanoat, 1,2-dimethoxy-1-phenyl-ethane, phytol, and stigmasterol. Fraction 4.3 is colorless needle-shape crystals and identified as 8-acetate-7-phenyl-2,6-dioxabicyclo[3.3.1]nonane-3-one or 9-deoxygoniopypyrone acetate. Diameters of inhibition zone of methanol extracts, n-hexane, and ethyl acetate with concentration of 5% against Staphylococcus aureus bacteria are 7.40, 2.50 and 6.18 mm respectively, whereas against Escherichia coli are 6.63, 2.46 and 4.07 mm respectively. Diameters of inhibition zone of 9-deoxygoniopypyrone acetate with concentration of 0.2% against Staphylococcus aureus and Escherichia coli are 6.27 mm and 5.25 mm respectively