ANALISIS FARMAKOLOGI DAN EPIDEMIOLOGI RESIDU DELTAMETRIN PADA DAGING BROILER DI WILAYAH KABUPATEN SLEMAN DAERAH ISTIMEWA YOGYAKARTA

• Main Authors: , R. Gagak Donny Satria, , Prof. Dr. drh. Bambang Sumiarto, S.U., M.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/130732/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71161
• Institution: Universitas Gadjah Mada

Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from Pesticide residue in the meat and liver of broiler causes troubles for consumers in getting safe, healthy, whole, and halal nutrients. This research was aimed to carry out an optimization and validation of deltamethrin analysis procedure by using high performance liquid chromatography (HPLC), based on specification of parameter, precision, accuracy, limit of quantification, linearity, and confirmation of the compliance level by using Kappa test. The other aim of this research was to carry out the pharmacology experiment on deltamethrin residue in the meat of broiler that had been fed with different dosages of deltamethrin orally and analyzed its correlation between treatment and concentration found in the specimens. Research on prevalence and situation description of deltamethrin residue in meat of broiler at the district of Kabupaten Sleman, including the the possible associated factors, was carried out by using epidemiology study. The main equipment used for the residue analysis in tissue was HPLC with the appropriate procedure. The research consisted of the stage of validation and suitability analysis, experimental stage, and field research. Samples that were used for validation analysis were taken from markets located around Yogyakarta. Experiment was conducted by using 25 broilers as experimental animals. Total of 512 broiler samples for field research, were taken by using double-stage method from different locations at the district of Kabupaten Sleman. Results of this research showed that deltamethrin analysis procedure using HPLC was optimal and had good validity. Analysis procedure has been qualified for the main parameter validations. The validity analysis was supported by the analysis result of Kappa suitability with good categories (Kappa = 0,68). Oral exposure of deltamethrin through drinking water showed that there was correlation between deltamethrin treatment and residue in the blood, liver, and meat. The prevalence of deltamethrin residue was 17,6% with the mean of positive samples that contained deltamethrin 0,709 mg/kg. The Factors associated with increase the likelihood of positive samples of meat residue were animal health management (OR = 191,066), types of farm bedding (OR = 14,912), lacking of business experience(OR = 11,361), farms in the southeast region (OR = 11,269), the usage of ground water (OR = 10,351), unideally disinfection program of warehouses (OR = 6,092), limitation access to the farm (OR = 5,840), and presence of deltamethrin in the water (OR = 2,098). The Factors associated with decrease the likelihood of positive samples of meat residue were the location of farms in the northeast region (OR = 0,007), the sanitation program incoming vehicles (OR = 0,121), proportional arrangement of workers number (OR = 0,126), controlling farm�s environment (OR = 0,146), the presence of deltamethrin in the liver (OR = 0,202), open system of water installation (OR = 0,226), chicken�s population in the farm > 10.000 (OR = 0,364), postal type of broiler�s house (OR = 0,455), veterinarian as farm�s manager (OR = 0,485). Factors that associated and increased meat residue�s level were vehicle disinfection program (coefficient = 0,439), flexibility of working arrangements (coefficient = 0,261), presence of deltamethrin in the water from the environment (coefficient = 0,223), chicken�s population in the farm > 10.000 (coefficient = 0,208). Factors that were associated and decrease meat residue�s level were restriction of access (coefficient = -0,629), main job of workers (coefficient = -0,316), Veterinarian or engineers of as farm�s manager (coefficient = -0,274), and chicken period < 35 days (coefficient = -0,242). The research concluded that HPLC procedure was valid to analyze deltametrin. Deltametrin residue in boiler�s meat was caused by external factors. The prevalence of broiler meat residue on Sleman was 17,6%, contained deltamethrin 0,709 mg/kg in average, and influence by farm management and environmental factors.