KAJIAN SEROLOGI DAN MOLEKULER CRINIVIRUS PADA TANAMAN TOMAT

• Main Authors: , RESTI FAJARFIKA, , Dr.Ir. Sedyo Hartono, M.P.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/130868/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71301
• Institution: Universitas Gadjah Mada

Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) are whitefly vectors-transmitted virus, members of the family Closteroviridae, genus Crinivirus that causes yellowing which has been causing serious production problems in tomato crops, production is affected through decreased fruit size and numbers yield. This research aimed to detect the ToCV and TICV in tomato plant by serological and molecular detection and knowing the detection methods that specific, accurate, fast, and simple. The sample of disease plant was collected from Kopeng and Magelang (Central Java), Indonesia. Five methods are serological (DTBIA, IEM, and IC-PCR) and molecular methods (SDT-RT-PCR, and RT-PCR from commercial kit) were using specific polyclonal antibodies and primers of TICV and ToCV. The result of IEM methods was found two types of particles viruses which flexuous filamentus. Particle lengths of ToCV and TICV were 810.69 nm and 872.34 nm, respectively. The positive reaction of DTBIA method indicated purple or magenta discoloration in the phloem limited of top, bottom, side shoots, and yellow curly symptoms on plants and healthy plants remained green, no color change. IC-PCR, SDT-RT-PCR, and RT-PCR methods were successfully amplified TICV DNA from Magelang isolate, 416 bp size. Blast analysis of nucleotide and amino acid sequences to make sure that Crinivirus species is TICV. The ToCV species was detected with IEM and DTBIA methods, where as the TICV species by the IEM, DTBIA, IC-PCR, SDT-RT-PCR, and RT-PCR. The fifth method is specific detection tool, the IC-PCR have higher accuracy, the DTBIA more practical and cheper, the IEM have faster but expensive. The SDTRT- PCR methods are simpler, faster, and cheper than RT-PCR to gain the total RNA. Based from the nucleotide sequencing and amino acid gene CPd, the Magelang isolate that infected in tomatoes was detected as TICV that include in the GenusCrinivirus, Family Closteroviridae. Key words: Yellowing diseases, Tomatoes, TICV, ToCV, Serological detection, Molecular detection