GAMBARAN LESI MAKROSKOPIS DAN MIKROSKOPIS EMBRIO AYAM YANG DIINFEKSI VIRUS Infectious Bursal Disease (IBD)

• Main Authors: , GUSTIN MAHMUDAH, , Dr. drh. Michael Haryadi Wibowo, MP.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/130910/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71343
• Institution: Universitas Gadjah Mada

Infectious Bursal Disease (IBD) or well known as Gumboro disease is a disease of poultry that is often found in the field where significant losses result. Accurate diagnosis of the disease is needed in efforts to control the disease, one of which is the isolation and identification of viruses that causes disease. This research was aimed to determine the macroscopic and microscopic lesions in chickens infected by the virus of IBD. The virus isolate from the collection of Dr. drh. Michael Haryadi Wibowo, M. P and has been identified by Transciptase Reverse Polymerase Chain Reaction (RT-PCR). This research was used embryonated chicken eggs 11 day old and antibody negative to IBD. Embryonated chicken eggs was used three eggs which are divided into a control group and infected group. IBD virus inoculation was did by deposition on the chorioallantoic membrane (CAM). Embryos were monitored for mortality and observed macroscopically on CAM and embryos. The organs of chicken embryos were made into histopathologic preparations stained with Hematoxyline and eosin (H&E) for microscopic analysis. The results showed that the negative control of chicken embryos and CAM had no any change. The inoculated IBD virus isolate showed macroscopic lesions cutaneous congestion, hemorrhages on toe joints and the cerebral region. Embryos were observed edematous distention of abdominal region, delayed feathering, embryo development was stunted and CAM was hemorrhagic. The microscopic lesions showed congestion, vacuolization and cell death in the liver, tubular epithelial necrosis in kidney. The others microscopic lesions embryos were found hemorrhagic, vacuolization, and there is infiltration of lymphocytes in the heart, skin udema, congestion in lung and hemorrhages on CAM and brain.