VALIDASI PROSEDUR ANALISIS DELTAMETHRIN PADA DAGING AYAM BROILER MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT)

• Main Authors: , PRADITA RENDYAWAN, , drh. R. Gagak Donny Satria, M.P., M.Pd.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/131360/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71816
• Institution: Universitas Gadjah Mada

Analysis technology of pesticide residues more develop in a row with increases usage pesticide in a field. There are several of residues analysis method, one of them is HPLC. This research aims to perform validate posseses of analysis deltamethrin using a Shimadzu 6.1 high-performance liquid chromatography (HPLC) with control system SCL-10 A VP, DGU-14 A degasser. Mobile phase of Merck acetonitrile 80% in steryl aquadest, flow rate of 1.25 ml/minute, UV-Vis SPD 10 AV VP detector with wavelength of 236 nm and used Shimadzu Shim-pack 150 L x 4.6 mm VP-ODS Column C18 on temperature 30 oC. The validation examination is based on specificity, precision, accuracy, limit of qualitative, quantitative limits, and linearity. Results of the research shows the average of area concentration 1 μg/ml, 2 μg/ml, and 5 μg/ml in consecutive are 34620.33, 79048.67, dan 181208.7 with retention time in 8.6-8.98 minute. Repeatability is clone 3 times and the RSD mean value obtained is � 2%. The detection limit at 0.1 μg/ml and limit of quantification at concententration 1 μg/ml. The equation of linear line y = 36046x+2182, with the value of R= 0.99. It can be concluded that the method used in the research posseses good validity.