EFFECT OF FLAT NOSE PITVIPER (Trimeresurus puniceus) VENOM ON THE MORPHOLOGY AND THE PATTERN OF AGGREGATION AND HEMOLYSIS ON COW BLOOD IN VITRO

• Main Authors: , BOO BIING JYE, , Dr. drh. Yanuartono, MP.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/132071/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72587
• Institution: Universitas Gadjah Mada

Trimeresurus puniceus is a viper that can be found on Java and southern Sumatra. Snake envenomation an important and often neglected source of human suffering especially in developing country including Indonesia. Currently there is no monovalent anti-venom and effect treatment for patient bitten by Trimeresurus puniceus. The objective of this research is to study the effect of Trimeresurus puniceus venom on the morphology and the aggregation and hemolysis of cow�s red blood cell. The materials used in this research are the venom of Trimeresurus punicues and cow erythrocyte. The research was carried out by diluting the venom of Trimeresusrus puniceus with phosphate buffer saline (PBS) into concentrations of 10%, 1% and 0.1% and each concentration was mixed with 100% cow erythrocyte in 1:1 ratio, then rest it for 5 minutes. The red blood cell morphology was examined with a microscope and the pattern of aggregation and henolysis was estimated with red blood cell number using a hemocytometer. All the red blood cell morphology undergoes multiple changes including changing of biconcave shape to spherocytes, aniscocytosis, and echinocytes. The venom of Trimeresurus puniceus cause aggregation and hemolysis in red blood cell in all the 3 concentration that we tested, with roughly 73% aggregation and hemolysis in venom with 10% concentration. It was concluded that the venom of flat nose pit viper (Trimeresurus puniceus) has high hemolytic action and the effect is higher with the increase of venom concentration. Keyword: venom, Trimeresurus puniceus, erythrocyte, morphology, aggregation, hemolysis,cow