BIOAKTIVITAS N,O-KARBOKSIMETIL KITOSAN TERHADAP BAKTERI PATOGEN PADA IKAN BUDIDAYA
Chitosan has been known as an antibacterial agent which is biodegradable, biocompatible, but insoluble in neutral solvents. Modification into N,O-carboxymethyl chitosan (N,O-CMC) was done in order to increase solubility in neutral solvent without changing its bioactivity so application as an antibac...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/132101/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72617 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Gadjah Mada |
| Summary: | Chitosan has been known as an antibacterial agent which is biodegradable, biocompatible, but
insoluble in neutral solvents. Modification into N,O-carboxymethyl chitosan (N,O-CMC) was
done in order to increase solubility in neutral solvent without changing its bioactivity so
application as an antibacterial agent against fish disease bacteria is allowed. Three methods
were employed to produce N,O-CMC. Characterization was done in order to know the
important characteristics of chitosan and its derivate, i.e. yield, solubility, chemical bond and
degree of deacetylation. The result in characterization showed increasing in solubility and
degree of deacetylation. The three products were tested against fish pathogens Aeromonas
hydrophila, Streptococcus sp. and Vibrio spp. Two types of N,O-CMC products have
antibacterial activity against Streptococcus sp., and Vibrio spp. None of N,O-CMC products
showed antibacterial activity against Aeromonas hydrophila. The best concentration of Liu�s
N,O-CMC and Hayes�s N,O-CMC against bacteria in this experiment is 20 mg/mL.
Keywords : antibacterial activity, chitosan, N,O-carboxymethyl chitosan, fish disease bacteria,
MIC. |
|---|