A STUDY ON THE EFFECTS OF THE FLAT NOSED PIT-VIPER (Trimeresurus puniceus) VENOM ON CANINE ERYTHROCYTES

• Main Authors: , DANIEL SAMRAJ WILFRED, , Dr. drh. Yanuartono, MP.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/132903/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73448
• Institution: Universitas Gadjah Mada

Trimeresurus Puniceus or also known as the Ashy Pit Viper, Flat-nosed Pit Viper or Javanese Pit Viper is local to the Java islands and South Sumatra. The venom of the Javanese pit viper is predominantly composed of hemotoxin�s that affect the victim�s circulatory system and also includes local necrosis, excessive bleeding and also severe inflammation. The venom concentration to cause a fatality is still unknown. The main purpose of this study is to document and further understand the effect of T.puniceus's venom on dog erythrocytes, also to understand the venom's effectiveness with varied concentrations. The experiment is conducted in vitro, the venom was collected using standard venom collecting procedures and then dried to form crystals. The venom is then diluted with PBS (Phosphate Buffer Solution) to form concentrations of 0.1% and 10%. Combining canine blood with equal parts of venom the smear was then viewed after 10 seconds and evaluated microscopically, this process was repeated 6 times. The erythrocyte destruction (hemolysis and enchinocytosis) increased as the concentration was increased. Both concentrations were compared to the control and to each other. The conclusion drawn is that hemolysis at a significant level can be caused by the ashy pit viper�s venom at concentrations below 10%.