EKSPRESI GEN RFT1 DALAM SIKLUS DIURNAL MASA TRANSISI PEMBUNGAAN TANAMAN PADI HITAM (Oryza sativa L. �Cempo Ireng�)

• Main Authors: , FARIDA NUR MALIKA, , Dr. Yekti Asih Purwestri, S.Si., M.Si.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/133079/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73634
• Institution: Universitas Gadjah Mada

In Indonesia, there are a lot of local rice cultivars, one of them is black rice cultivar Cempo Ireng. In rice, there are 13 FT-like genes that have been identified (OsFTL1-OsFTL13). OsFTL3/RFT1 is one of the most active gene involved in flowering induction of rice. RFT1 gene expression of black rice that have been evaluated before, showed that temporal expression of RFT1 was most clearly viewed at 70 until 75 days after planting (DAP). The objective of this research was to evaluate the RFT1 gene expression in the daily/diurnal cycle during the flowering transition phase. Research method consists of RNA isolation, determination of RNA quality, cDNA formation, RFT1 gene amplification, and RFT1 gene expression�s quantification. RNA was isolated from black rice leaf of 70 and 90 DAP, taken at 08.00, 12.00 and 16.00 o�clock. Sample was isolated using RNeasy Plant Mini Kit. RNA quality was determined by spectrophotometer at λ = 260 nm and 280 nm. The cDNA was synthetized by two-step RT-PCR and cDNA quality was checked by Ubiquitin gene amplification as the internal control. The result of Ubiquitin and RFT1 amplification were visualized using agarose gel electrophoresis. Quantification of RFT1 and Ubiquitin expression were conducted using ImageJ program to estimate RFT1 and Ubiquitin concentration based on the width and density of bands on the electrophoresis result. The RNA had a good quality with the ratio of 1.9-2.3. Electrophoresis result showed that Ubiquitin gene was expressed in all samples with approximately 200 bp in size. RFT1 gene of black rice was expressed in all samples with approximately 200 bp in size. RFT1 expression level at 08.00 o�clock was about 2.5-3 times higher than the expression at 12.00 and 16.00 o�clock.