EFEK IMUNOMODULATOR DUA SENYAWA NEOLIGNAN HASIL ISOLASI DARI EKSTRAK METANOL DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.): Kajian Imunitas Seluler dan Humoral

• Main Authors: , Yustina Sri Hartini, , Prof. Dr. Subagus Wahyuono, M.Sc., Apt.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/133782/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74600

Red betel (Piper crocatum) leaf is widely used as a traditional medicine in Indonesia. Some of the pharmacological activities of red betel leaf extract have been reported. The in vivo study reported that red betel leaf extract had macrophage phagocytic activity but had no significantly effect on lymphocyte proliferation and immunoglobulin G titre. In vitro study of two isolated compounds (1 and 2) from red betel leaf extract showed that at the dose of 5 μg/ml increased macrophage phagocytic activity. The 1 dan 2 were identified as neolignans, i.e., 2-allyl-4-(1'- hydroxy-1'-(3 \", 4\", 5 \"-trimethoxyphenyl) propan-2'-yl) -3,5-dimethoxycyclohexa- 3, 5-dienone and 2-allyl-4-(1'-acetyl-1'-(3 \", 4\", 5 \"-trimethoxyphenyl) propan-2'- yl) -3,5-dimethoxycyclohexa- 3,5-dienone. This study aim to determine the in vivo immunomodulatory and histopathological features effect of 1 dan 2 on mice. Isolate 1 and 2 were tested on BALB/c mice immune response induced by Listeria monocytogenes. The 1 and 2 cellular immune response determined through macrophage phagocytic activity test, nitric oxide production, and lymphocyte proliferation, whereas the compounds effect on the humoral immune response determined by measuring imunoglobulin G titre. Furthermore, 1 and 2 effect on the IL-12 and IL-10 productions were also determined. Histopathological features effects of 1 and 2 were observed from the liver and kidney which made by hematoxyllin-eosin staining, and observed microscopiccally. The data of immunomodulatory effects were analysed using one-way ANOVA followed by Tukey test, while the liver and kidney histopathological features were analysed descriptively. This in vivo study show that 1 and 2 (5 mg/kg body weight) increase the phagocytic activity of macrophages. At the doses of 2.5, 5, and 10 mg/kg body weight, 1 and 2 increase the production of nitric oxide and interleukin 12 but the mice lymphocyte proliferation, immunoglobulin G titre, and interleukin 10 production have no significantly differences compare to control. The treatment with 1 (2.5, 5, and 10 mg/kg body weight) has no histopathological features effect on both liver and kidney, but at the dose of 2.5 and 5 mg/kg body weight 2 induce liver hidropic degeneration and necrosis respectively.