MIKROSPOROGENESIS PADA Crinum asiaticum L. DAN Hymenocallis littoralis (Jacq.) Salisb. (Amaryllidaceae): STUDI ANATOMI DAN ULTRASTRUKTURAL

• Main Author: WARDHANA, HENDRA
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2015
• Subjects: Microbiology; Biological Sciences
• Online Access: https://repository.ugm.ac.id/134825/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=77987
• Institution: Universitas Gadjah Mada

Anther and pollen development of Crinum asiaticum L. and Hymenocallis littoralis (Jacq.) Salisb. was observed using Light Microscope (LM) and Scanning Electron Microscope (SEM). The anthers were collected from different size of flower buds that represented development stages. For LM observation, the anthers were fixed with FAA and cross-cut with paraffin method. For SEM observation, the anthers were cross-cut using free hand method with 5 mm thickness. The anthers were observed with JEOL JSM T-300 SEM microscope. The result showed that the anthers of both species were tetrasporangiate with tissues compose of epidermis, endotechium, middle layer, and tapetum layer. The anther wall of C. asiaticum L. had epidermal protrusion, three layers of middle layer tissue, and secretory/glanduler tapetum. The anther wall of H. littoralis (Jacq.) Salisb. had oval epidermis, two layers of middle layer tissue, and amoeboid/non-glanduler tapetum. The endotechium of both species developed to fibrous thickening cells . Orbicule was found in the anther of C. asiaticum L., but was not found in H. littoralis (Jacq.) Salisb.. The pollen type of C. asiaticum L. and H. littoralis (Jacq.) Salisb. was monosulcate. The pollen wall ornamentation of C. asiaticum was small spines (spinula) and was reticulate in H. littoralis (Jacq.) Salisb.. The pollen nucleus division of H. littoralis (Jacq.) Salisb. occurred before the anther dehiscence and the pollen was out from the anther.