Peranan Transforming Growth Factor β-1 (TGF β-1) dalam Perkembangan Penyakit Jantung Akibat Induksi Diet Lemak Tinggi
Abstract Cardiovascular diseases are the disorder of artery carrying blood into heart muscle and characterized by thickening, stiffening and constriction of the artery. In spite of cardiovascular disease cases have been known since along time ago, the main factor, mechanism, pathogenesis and indi...
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| Main Authors: | , |
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| Format: | Article PeerReviewed |
| Language: | English |
| Published: |
Fakultas Kedokteeran Hewan
2012
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/135019/1/ENDAH%20JSV%20JULI%202012%20artikel%202.pdf https://repository.ugm.ac.id/135019/ |
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| Institution: | Universitas Gadjah Mada |
| Language: | English |
| Summary: | Abstract
Cardiovascular diseases are the disorder of artery carrying blood into heart muscle and characterized by
thickening, stiffening and constriction of the artery. In spite of cardiovascular disease cases have been known
since along time ago, the main factor, mechanism, pathogenesis and indicator of the early existence of these
diseases are still unknown. Transforming growth factor β-1 (TGF β-1) is a growth factor produced by various cell
types which are presumed to be involved in cardiovascular diseases. This research was done to find out the role of
TGF β-1 in cardiovascular diseases. Twenty female Sprague Dawley rats, 150 grams of body weight were used in
this research. Rats were adapted for seven days, and then divided randomly into two Groups, 10 rats for each.
Group I was fed in standard diet containing 5% of saturated fat, while Group II was fed in high fat diet containing
30% of saturated fat. Rats were maintained for two months, corresponding to each diet treatment of both groups
and water ad libitum. After two months of the diet, blood was collected from orbital vein and stored into
uncoagulated tube for triglyceride measurement. Rats were then killed, neropsied and their heart tissues were
collected into tubes containing 10% formaldehyde for histopathologic analysis and immunohistochemistry to
see the expression of TGF β-1. The result of blood analysis showed that the average of triglyceride concentration
of Group I was 126 mg/dl and 228.1 mg/dl of Group II. After statistically analyzed using student t-test, it showed
that there was a significant difference between Group I and II (P<0,05). Eventhough histopathologic analysis of
heart tissue did not show any alteration, the TGF β-1 was expressed in all heart tissue of Group II . Based on the
results above, it can be concluded that TGF β-1 is involved in the incidence of cardiovascular disease and it is
possible to use TGF β-1 as a diagnosticmarker of cardiovascular disease.
Keywords : heart, cardiovascular disease, orbital vein, high fat diet, TGF β-1 |
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