Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells

• Main Authors: Susilowati, Heni, Arifani, Maria, Handajani, Juni
• Format: Article PeerReviewed
• Language: English
• Published: 2012
• Subjects: Cellular Immunology
• Online Access: https://repository.ugm.ac.id/135911/1/no%2012%20-%202nd%20IJS%20Oral%20n%20Dent%20Sc%20hal%20394-398.pdf; https://repository.ugm.ac.id/135911/
• Institution: Universitas Gadjah Mada

Calcium hydroxide [Ca(OH)2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OH)2 is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The effect of Ca(OH)2 in NO regulation in pulp tissue cells is still unknown. As fibroblast is an important component in pulp tissue, the aim of this study was to investigate whether NO production is affected by Ca(OH)2 stimulation in fibroblast cells. Vero, a fibroblast-like cell line (2 x 104) was cultured in M199 culture medium. Overnight culture were stimulated with various concentrations of Ca(OH)2 solution (12,5 mg/ml, 25 mg/ml, and 50 mg/ml), or left untreated as negative control, and then were incubated for 24 h at 37oC with 5% CO2. Nitrite from every sample was detected with Griess reaction. Data were analyzed using Kruskal-Wallis one-way Anova and Dunnet’s T3. The results demonstrated that 12,5 mg/ml and 25 mg/ml Ca(OH)2 caused similar effect on NO production, whereas the 50 mg/ml Ca(OH)2 could induce NO production in Vero cells. It was concluded that NO production might be modified and involved in the cellular change in dental pulp fibroblast under the Ca(OH)2 exposure.