Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells

Calcium hydroxide [Ca(OH)2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OH)2 is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The eff...

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Main Authors: Susilowati, Heni, Arifani, Maria, Handajani, Juni
Format: Article PeerReviewed
Language:English
Published: 2012
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Online Access:https://repository.ugm.ac.id/135911/1/no%2012%20-%202nd%20IJS%20Oral%20n%20Dent%20Sc%20hal%20394-398.pdf
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spelling id-ugm-repo.1359112016-08-05T06:52:35Z https://repository.ugm.ac.id/135911/ Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells Susilowati, Heni Arifani, Maria Handajani, Juni Cellular Immunology Calcium hydroxide [Ca(OH)2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OH)2 is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The effect of Ca(OH)2 in NO regulation in pulp tissue cells is still unknown. As fibroblast is an important component in pulp tissue, the aim of this study was to investigate whether NO production is affected by Ca(OH)2 stimulation in fibroblast cells. Vero, a fibroblast-like cell line (2 x 104) was cultured in M199 culture medium. Overnight culture were stimulated with various concentrations of Ca(OH)2 solution (12,5 mg/ml, 25 mg/ml, and 50 mg/ml), or left untreated as negative control, and then were incubated for 24 h at 37oC with 5% CO2. Nitrite from every sample was detected with Griess reaction. Data were analyzed using Kruskal-Wallis one-way Anova and Dunnet’s T3. The results demonstrated that 12,5 mg/ml and 25 mg/ml Ca(OH)2 caused similar effect on NO production, whereas the 50 mg/ml Ca(OH)2 could induce NO production in Vero cells. It was concluded that NO production might be modified and involved in the cellular change in dental pulp fibroblast under the Ca(OH)2 exposure. 2012-03-01 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/135911/1/no%2012%20-%202nd%20IJS%20Oral%20n%20Dent%20Sc%20hal%20394-398.pdf Susilowati, Heni and Arifani, Maria and Handajani, Juni (2012) Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells. Proceeding of The Second International Joint Symposium on Oral and Dental Sciences 2012. pp. 394-398. ISSN 978-602-9461-16-9
institution Universitas Gadjah Mada
building UGM Library
country Indonesia
collection Repository Civitas UGM
language English
topic Cellular Immunology
spellingShingle Cellular Immunology
Susilowati, Heni
Arifani, Maria
Handajani, Juni
Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
description Calcium hydroxide [Ca(OH)2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OH)2 is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The effect of Ca(OH)2 in NO regulation in pulp tissue cells is still unknown. As fibroblast is an important component in pulp tissue, the aim of this study was to investigate whether NO production is affected by Ca(OH)2 stimulation in fibroblast cells. Vero, a fibroblast-like cell line (2 x 104) was cultured in M199 culture medium. Overnight culture were stimulated with various concentrations of Ca(OH)2 solution (12,5 mg/ml, 25 mg/ml, and 50 mg/ml), or left untreated as negative control, and then were incubated for 24 h at 37oC with 5% CO2. Nitrite from every sample was detected with Griess reaction. Data were analyzed using Kruskal-Wallis one-way Anova and Dunnet’s T3. The results demonstrated that 12,5 mg/ml and 25 mg/ml Ca(OH)2 caused similar effect on NO production, whereas the 50 mg/ml Ca(OH)2 could induce NO production in Vero cells. It was concluded that NO production might be modified and involved in the cellular change in dental pulp fibroblast under the Ca(OH)2 exposure.
format Article
PeerReviewed
author Susilowati, Heni
Arifani, Maria
Handajani, Juni
author_facet Susilowati, Heni
Arifani, Maria
Handajani, Juni
author_sort Susilowati, Heni
title Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
title_short Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
title_full Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
title_fullStr Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
title_full_unstemmed Nitric Oxide Production Responsiveness to the Exposure of Calcium Hydroxide in Fibroblast Cells
title_sort nitric oxide production responsiveness to the exposure of calcium hydroxide in fibroblast cells
publishDate 2012
url https://repository.ugm.ac.id/135911/1/no%2012%20-%202nd%20IJS%20Oral%20n%20Dent%20Sc%20hal%20394-398.pdf
https://repository.ugm.ac.id/135911/
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