The Detailed Motility and Velocity Characteristics of Rams Spermatozoa as Assessed by Computer-Aided Semen Analysis

• Main Author: Ismaya, Ismaya
• Format: Conference or Workshop Item PeerReviewed
• Language: English
• Published: 2015
• Subjects: Animal Reproduction
• Online Access: https://repository.ugm.ac.id/136459/7/The_6th_ISTAP%20P%202015%20%28Ismaya-28%29.pdf; https://repository.ugm.ac.id/136459/
• Institution: Universitas Gadjah Mada

ABSTRACT: The aim of the present study was to determine the effect of incubation time in TALP medium on the detailed motility and velocity of spermatozoa characteristics. Four rams (R9, R12, R13, R16) of proven fertility were used in this study. Semen was collected by electroejaculation. Rams semen was collected with 4 replicates, respectively to analyzed the motility and velocity characteristics by using the computer-aided semen analysis (CASA). Data were analyzed using SPSS software program. The results showed that percentage of motile, progressive and rapid were significantly decreased from 0 to 120 minutes incubation. In contrast, percentage of static was significantly increased from 21.6% to 71.3% at 0 to 120 minutes incubation. Ram 12 had the best motile with 76.3% at 0 minute incubation and 40.3% at 120 minutes incubation. Average path velocity (VAP), progressive velocity (VSL) and track speed (VCL) at 0 incubation were 91.2 pm/s, 72.2 pm/s and 134 pm/s afterward decreased to 49 pm/s, 40.8 um/s and 77.7 pm/s at 120 minutes incubation, respectively. In conclusion, rams and incubation time were influenced the motility and velocity of spermatozoa. Keywords: Motility, Velocity, Rams Spermatozoa.