Genetic Diversity of Ongole Crossbred Cattle in Kebumen based on MC4R Gene

• Main Authors: Fathoni, Ahmad, Sumadi, Sumadi, Hartatik, Tety, Khusnudin, Mukhamad, Maharani, Dyah, Ngadiyono, Nono, Widayati, Diah Tri, Noviandi, Cuk Tri
• Format: Conference or Workshop Item PeerReviewed
• Language: English
• Published: 2016
• Subjects: Animal Production
• Online Access: https://repository.ugm.ac.id/139395/1/C18_Fathoni%20%282016%29_AAAP_Rev.pdf; https://repository.ugm.ac.id/139395/
• Institution: Universitas Gadjah Mada

The Melanocortin-4-receptor (MC4R) gene is known as an important candidate gene for the growth trait. The aim of this study was to determine genetic diversity of Ongole crossbred cattle in Kebumen based on MC4R gene analysis. Total sixty blood samples of Ongole Crossbred from Klirong district of Kebumen were collected for DNA isolation. The PCR product was amplified with forward primer 5’-GTCGGGCGTCTTGTTCATC-3’ and reverse primer 5’CTTGTGTTTAGCATCGCGT-3’. The single nucleotide polymorphisms (SNP1133C>G) base on NCBI position were obtained by sequencing methods. It was used for genotyping of the cattle by using PCR-RFLP methods with HpyCH4IV restriction enzyme. The result of PCR-RFLP indicated C and G alleles. The frequencies G allele (0,59) was greater than C allele (0,41). The highest genotype frequencies have been detected in CG heterozygote animals (0,52%) followed by GG (0,33) and CC (0,15) homozygote animals. Animals having homozygote CC were defined when the fragments size being recognized at 493, while homozygote GG was 173 and 320 bp. The heterozygote CG existed by PCR-RFLP method at the same position of the homologous chromosome with 173, 320 and 493 bp of fragments size. As a conclusion, the SNP 1133 C>G of MC4R gene may have been able to determine the genetic diversity of Ongole crossbreed cattle in Kebumen