Identification of"Methyl Formate-Producing Enzyme" in Cell-Free Extract of Pichia Methanolica = Identifikasi"enzim pembentuk metil formar" dari ekstrak sel Pichia methanolica
ABSTRACT: Cell-free synthesis of methyl formate by extract of Pichia methanolica was investigated. It was observed that the addition of formaldehyde could increase the methyl formate-producing activity compared to control experiment which contained methanol as a sole substrate for methyl formate pro...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
1993
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/17388/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=140 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Gadjah Mada |
| Summary: | ABSTRACT:
Cell-free synthesis of methyl formate by extract of Pichia methanolica was investigated. It was observed that the addition of formaldehyde could increase the methyl formate-producing activity compared to control experiment which contained methanol as a sole substrate for methyl formate production. On the other hand, the addition of formic acid, formate, and S-formylglutathione or GSH did not increase the activity. Methyl formate-producing activity disappeared upon gel filtration of the cell-free extract on Sephadex G-25. However, the full activity was recovered with the addition of NAD+. These results indicated that methyl formate-producing enzyme was present in cell-free extract. This enzyme was found to be responsible for methyl formate formation from methanol and formaldehyde when its cofactor (NAD+) was available. Based on this finding, the standard assay system for methyl formate-producing enzyme could be constructed. Purification of methyl formate-producing enzyme from cell-free extract was possible due to the availability of the standard assay system constructed in this experiment.
Keywords : identification |
|---|