Pengaruh kombinasi kadar sukrosa dan kalium nitrat dalam medium murashige skoog (ms) terhadapkadar atropina dan atau hiosiamina pada kultur kalus Datura stramonium L. var. stramonium =Combination Effect of Sucrose and ..

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2004
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/17586/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=345
• Institution: Universitas Gadjah Mada

This research was aimed to study combination effect of sucrose and potasium nitrate concentration within Murashige-Skoog (MS) medium on producing biomass and'atropine/hyoscyamine contents within callus culture of Datura stramonium var. stramonium. In the research, explant of Datura stramonium var. stramonium leaves, planted on MS medium applying the addition of plant growth regulator 2,4-D and kinetin, each of 1 ppm. Medium for producing alkaloids applied similar medium to one for callus induction with addition of combination sucrose concentration (30, 35, 40 and 45 g/L) respectively and potasium nitrate (12.5%, 25%, 37.5%, and 50% based on standart content of MS medium). The results suggested that MS medium added with 40 g/L sucrose and 37.5% potasium nitrate (S40I1375 medium) turned to be the most effective medium to produce biomass, while MS medium added with sucrose concentration of 40 g/L without the addition of potasium nitrate (S4oNo medium) was the most effective medium to produce atropine/ hyoscyamine. Keywords : callus culture, atropine/hyoscyamine, Datura stramonium L. var. stramonium.