Cytotoxic Effects of Protein Fraction Isolated from Curcuma mangga Val Rhizomes and Containing Ribosome-Inactivating Protein on Cancer Cell-Lines and Normal Cell.

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2004
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/17746/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=511
• Institution: Universitas Gadjah Mada

C. manqqa Val. has been used as an alternative remedy for cancer in Yogyakarta. The protein fraction of C. manqqa was identified to contain Ribosome-inactivating Protein which cleave supercoiled double stranded DNA in vitro. In this experiment, the protein fraction isolated from fresh, 40°C dried and freeze dried C. manqqa Val. rhizome was screened against HeLa, Rail cell-lines and normal mononuklear cells for cytotoxic effects. This would enable us to describe the sensitivity of the protein extract on different cell types. The level of cytotoxicity was determined on the level of LC50 which was based on the percentage of the cell death following the 24 hours incubation with the extract. The protein isolated from C. manqqa VaL was able to cleave supercoiled double stranded DNA to nick circular form. This result suggested that protein contained RIPs. The highest activity was identified in the protein isolated from fresh C. manage Val, and this was followed respectively by freeze drying and 40°C drying C. manqqa VaL The comparison of the cytotoxic effect showed that protein of fresh C. manqqa Val produced the largest number of death cells and the most toxic was on the HeLa cell line. Moreover, the LC50 indicated that the highest cytotoxic effect was shown by protein isolated from fresh C. manqqa VaL followed respectively by freeze drying and 40°C drying C. manqqa Val. Based on LC50, the highest cytotoxic effect of C. manqqa Val was found on HeLa cell line, while similar cytotoxic effect was appear on Raji cell line and normal mononuclear cells Keywords: C. manqqa Val., RIP, cytotoxic, cancer cell lines, normal cell