Comparison of methods for genomic DNA isolation of rhizospheric microorganisms

Comparison of methods for genomic DNA isolation of rhizospheric microorganisms

A comparative study for the isolation of genomic DNA of rhizospheric microorganisms is described. The methods tested were based upon the methods of: (1) standard glass rod, (2) boiling glass rod, (3) Promega's Wizard genomic DNA isolation method, and (4) agarose gel plug, with some modification...

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Main Author: Perpustakaan UGM, i-lib
Format: Article NonPeerReviewed
Published: [Yogyakarta] : Universitas Gadjah Mada 1999
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Jurnal i-lib UGM
Online Access:https://repository.ugm.ac.id/17763/
http://i-lib.ugm.ac.id/jurnal/download.php?dataId=528
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Institution: Universitas Gadjah Mada
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spelling id-ugm-repo.177632014-06-18T00:34:58Z https://repository.ugm.ac.id/17763/ Comparison of methods for genomic DNA isolation of rhizospheric microorganisms Perpustakaan UGM, i-lib Jurnal i-lib UGM A comparative study for the isolation of genomic DNA of rhizospheric microorganisms is described. The methods tested were based upon the methods of: (1) standard glass rod, (2) boiling glass rod, (3) Promega's Wizard genomic DNA isolation method, and (4) agarose gel plug, with some modifications of each method. The efficiency and suitability of the method for genomic DNA isolation were analysed based on three parameters : (1) the level of DNA purity, (2) the concentration of DNA, and (3) the intactness of the DNA. It was observed that the standard glass-rod method gave the best results in terms of DNA purity and the DNA concentration obtained. However, in terms of DNA intactness, agarose gel plug method gave the best result compared to other methods. Keywords: genomic DNA - rhizospheric microorganisms [Yogyakarta] : Universitas Gadjah Mada 1999 Article NonPeerReviewed Perpustakaan UGM, i-lib (1999) Comparison of methods for genomic DNA isolation of rhizospheric microorganisms. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=528
institution Universitas Gadjah Mada
building UGM Library
country Indonesia
collection Repository Civitas UGM
topic Jurnal i-lib UGM
spellingShingle Jurnal i-lib UGM
Perpustakaan UGM, i-lib
Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
description A comparative study for the isolation of genomic DNA of rhizospheric microorganisms is described. The methods tested were based upon the methods of: (1) standard glass rod, (2) boiling glass rod, (3) Promega's Wizard genomic DNA isolation method, and (4) agarose gel plug, with some modifications of each method. The efficiency and suitability of the method for genomic DNA isolation were analysed based on three parameters : (1) the level of DNA purity, (2) the concentration of DNA, and (3) the intactness of the DNA. It was observed that the standard glass-rod method gave the best results in terms of DNA purity and the DNA concentration obtained. However, in terms of DNA intactness, agarose gel plug method gave the best result compared to other methods. Keywords: genomic DNA - rhizospheric microorganisms
format Article
NonPeerReviewed
author Perpustakaan UGM, i-lib
author_facet Perpustakaan UGM, i-lib
author_sort Perpustakaan UGM, i-lib
title Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
title_short Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
title_full Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
title_fullStr Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
title_full_unstemmed Comparison of methods for genomic DNA isolation of rhizospheric microorganisms
title_sort comparison of methods for genomic dna isolation of rhizospheric microorganisms
publisher [Yogyakarta] : Universitas Gadjah Mada
publishDate 1999
url https://repository.ugm.ac.id/17763/
http://i-lib.ugm.ac.id/jurnal/download.php?dataId=528
_version_ 1681217141648392192

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