Quick detection of citrus greening by PCR method with specific and universal primers

Huanglungbin or Greening is caused by ploem restricted unculturable bacterium, identified as Liberobacter. In general, Liberobacter found in Indonesia is Liberobacter asiaticum. The aim of this research was to develop the effective primers for Polymerase Chain Reaction (PCR) method in order to detec...

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Main Author:	Perpustakaan UGM, i-lib
Format:	Article NonPeerReviewed
Published:	[Yogyakarta] : Universitas Gadjah Mada 1999
Subjects:	Jurnal i-lib UGM
Online Access:	https://repository.ugm.ac.id/17773/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=539
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Institution:	Universitas Gadjah Mada

id	id-ugm-repo.17773
record_format	dspace
spelling	id-ugm-repo.177732014-06-18T00:36:04Z https://repository.ugm.ac.id/17773/ Quick detection of citrus greening by PCR method with specific and universal primers Perpustakaan UGM, i-lib Jurnal i-lib UGM Huanglungbin or Greening is caused by ploem restricted unculturable bacterium, identified as Liberobacter. In general, Liberobacter found in Indonesia is Liberobacter asiaticum. The aim of this research was to develop the effective primers for Polymerase Chain Reaction (PCR) method in order to detect citrus greening quickly. Primers used in this research were specific Primers (011, 012c, 0A1) and universal Primers (fDl/rPI). The specific Primers (011, 012c) were more effective and specific in detecting citrus greening than those of universal Primers (fDlirP1). In addition, the advantage of specific primers was that it could be used to amplify DNA greening of 1160 by only, but could not be used to amplify DNA of other sistemic pathogens. Keywords: Huanglungbin (HLB) â�� liberobacter â�� polymerase chain reaction â�� quick detection â�� specific and universal primers [Yogyakarta] : Universitas Gadjah Mada 1999 Article NonPeerReviewed Perpustakaan UGM, i-lib (1999) Quick detection of citrus greening by PCR method with specific and universal primers. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=539
institution	Universitas Gadjah Mada
building	UGM Library
country	Indonesia
collection	Repository Civitas UGM
topic	Jurnal i-lib UGM
spellingShingle	Jurnal i-lib UGM Perpustakaan UGM, i-lib Quick detection of citrus greening by PCR method with specific and universal primers
description	Huanglungbin or Greening is caused by ploem restricted unculturable bacterium, identified as Liberobacter. In general, Liberobacter found in Indonesia is Liberobacter asiaticum. The aim of this research was to develop the effective primers for Polymerase Chain Reaction (PCR) method in order to detect citrus greening quickly. Primers used in this research were specific Primers (011, 012c, 0A1) and universal Primers (fDl/rPI). The specific Primers (011, 012c) were more effective and specific in detecting citrus greening than those of universal Primers (fDlirP1). In addition, the advantage of specific primers was that it could be used to amplify DNA greening of 1160 by only, but could not be used to amplify DNA of other sistemic pathogens. Keywords: Huanglungbin (HLB) â�� liberobacter â�� polymerase chain reaction â�� quick detection â�� specific and universal primers
format	Article NonPeerReviewed
author	Perpustakaan UGM, i-lib
author_facet	Perpustakaan UGM, i-lib
author_sort	Perpustakaan UGM, i-lib
title	Quick detection of citrus greening by PCR method with specific and universal primers
title_short	Quick detection of citrus greening by PCR method with specific and universal primers
title_full	Quick detection of citrus greening by PCR method with specific and universal primers
title_fullStr	Quick detection of citrus greening by PCR method with specific and universal primers
title_full_unstemmed	Quick detection of citrus greening by PCR method with specific and universal primers
title_sort	quick detection of citrus greening by pcr method with specific and universal primers
publisher	[Yogyakarta] : Universitas Gadjah Mada
publishDate	1999
url	https://repository.ugm.ac.id/17773/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=539
_version_	1681217143540023296

Quick detection of citrus greening by PCR method with specific and universal primers

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