Meiotic chromosome of Pyricularia oryzae studied by fluorescence microscope using DAPI (4-6-diamindino-2-phenylindole) and G-banding method

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 1999
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/17800/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=568
• Institution: Universitas Gadjah Mada

Meiotic chromosome of Pyricularia oryzae were described using DAPI staining and G-banding method. With DAPI technique we liave succeeded in observing chromosomes of this fungi at the sexual stage, but this technique failed to stain the sexual stage of this fungi. By using G-banding chromosome of Pyricularia oryzae showing the typical G-band at prometaphase stage could be observed. The G-banding pattern between homologous chromosomes were apparently consistent, although the chromosome number 1 and 2 assumed as the shortest chromosome (1.8 urn). The banding pattern ofnos 3 and 4 (2.3 urn) are quite similar to each other and also to chromosome nos 5 and 6 (2.1 urn). Keywords: Pyricularia oryzae -DAPI -G-banding