Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody

Abstract Monoclonal antibodies were produced against mycelia and extra cellular proteins of Ustilago scitaminea and used for detection of infected sugarcane collected from Pengasih and Prambanan. Fractionation of mycelia and culture filtrat proteins were done with ammonium sulphate 85% and ultracent...

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Main Author:	Perpustakaan UGM, i-lib
Format:	Article NonPeerReviewed
Published:	[Yogyakarta] : Universitas Gadjah Mada 2002
Subjects:	Jurnal i-lib UGM
Online Access:	https://repository.ugm.ac.id/17951/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=726
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Institution:	Universitas Gadjah Mada

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spelling	id-ugm-repo.179512014-06-18T00:31:18Z https://repository.ugm.ac.id/17951/ Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody Perpustakaan UGM, i-lib Jurnal i-lib UGM Abstract Monoclonal antibodies were produced against mycelia and extra cellular proteins of Ustilago scitaminea and used for detection of infected sugarcane collected from Pengasih and Prambanan. Fractionation of mycelia and culture filtrat proteins were done with ammonium sulphate 85% and ultracentrifugation, were identified with the SDS-PAGE. The results indicated that mycelia and culture filtrat proteins of U. scitaminea had 50-141.435 kDa molecular weight. Four monoclonal antibodies: 26E4, 34G8,34G,, and 45C4 were specific for infected plants. The 26E4 was belong to IgM class of immunoglobulin, but the others was belonging IgG. All four monoclonal antibodies recognised U. scitaminea in the infected plants. Keywords: monoclonal - antibody - mycelia - culture filtrat [Yogyakarta] : Universitas Gadjah Mada 2002 Article NonPeerReviewed Perpustakaan UGM, i-lib (2002) Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=726
institution	Universitas Gadjah Mada
building	UGM Library
country	Indonesia
collection	Repository Civitas UGM
topic	Jurnal i-lib UGM
spellingShingle	Jurnal i-lib UGM Perpustakaan UGM, i-lib Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
description	Abstract Monoclonal antibodies were produced against mycelia and extra cellular proteins of Ustilago scitaminea and used for detection of infected sugarcane collected from Pengasih and Prambanan. Fractionation of mycelia and culture filtrat proteins were done with ammonium sulphate 85% and ultracentrifugation, were identified with the SDS-PAGE. The results indicated that mycelia and culture filtrat proteins of U. scitaminea had 50-141.435 kDa molecular weight. Four monoclonal antibodies: 26E4, 34G8,34G,, and 45C4 were specific for infected plants. The 26E4 was belong to IgM class of immunoglobulin, but the others was belonging IgG. All four monoclonal antibodies recognised U. scitaminea in the infected plants. Keywords: monoclonal - antibody - mycelia - culture filtrat
format	Article NonPeerReviewed
author	Perpustakaan UGM, i-lib
author_facet	Perpustakaan UGM, i-lib
author_sort	Perpustakaan UGM, i-lib
title	Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
title_short	Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
title_full	Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
title_fullStr	Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
title_full_unstemmed	Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody
title_sort	deteksi ustilago scitaminea pada tebu dengan antibodi monoklonal = detection o/ustilago scitaminea on sugarcane by monoclonal antibody
publisher	[Yogyakarta] : Universitas Gadjah Mada
publishDate	2002
url	https://repository.ugm.ac.id/17951/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=726
_version_	1681217177626083328

Deteksi Ustilago scitaminea Pada Tebu Dengan Antibodi Monoklonal = Detection o/Ustilago scitaminea on Sugarcane by Monoclonal Antibody

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