Preliminary report on allelic variation of Th2R and Th3R region of the circumsporozoite protein of plasmodium falciparum from Kokap Yogyakarta Province Indonesia

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2000
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/18074/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=851
• Institution: Universitas Gadjah Mada

Allelic variation on the Th2R and Th3R non-repetitive epitope regions of the Circumsporozoit Protein (CSP) is a well known phenomenon among Plasmodium falciparum population within different regions or geographical situations. However, such information originated from Indonesian plasmodium population is still not available. Studies have been initiated on Indonesian Plasmodium populations, aiming to identify the sequence variation of the gene encoding Th2R and Th3R epitope region of the CSP molecules of P. falciparum from malaria endemic areas of Indonesia. At the initial stage of these studies P. falciparum infected blood had been collected from patients living in endemic areas of Kokap district, Yogyakarta Province, Indonesia. Genomic DNA were isolated from microscopically positive samples and the gene encoding non-repetitive T cell epitope region of the CSP molecules were amplified by Polymerase Chain Reaction (PCR) using primers designed based on conserved regions of published sequences. The DNA PCR amplification products were purified and sequenced directly using ABI Prism 377 type Automatic DNA Sequencer, and the allelic type of the Th2R and Th3R epitopes were analyzed. The parallel PCR products were also dotted onto replicate nylon membranes and the Th2R and Th3R epitopes were typed using Sequence Specific Oligonucleotide Probes. The results indicated that from 18 samples which were successfully sequenced the Th2R epitope types were all Th2R*05 (100%), whiles on the Th3R locus 72.2% were Th3R*01