Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals
Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction...
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Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2000
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Online Access: | https://repository.ugm.ac.id/18117/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=895 |
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Institution: | Universitas Gadjah Mada |
Summary: | Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction patterns among four 2,4-D-degrading bacteria, two genotypes (I and II) were obtained. Based on 16S rDNA sequence analysis, those bacteria were identified as Vibrio natriegens and Vibrio alginilyticus.
Keywords: 2,4-D-degrading bacteria � 16S rDNA � PCR � RFLP � phylogenetic |
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