Kloning dan ekspresi gen S penyandi hepatitis b surface antigen (hbsag) virus hepatitis b � isolate wiatarawi, lowibok=Cloning and Statement of the S Gene Encoding the Hepatitis B Surface Antigen (HBsAg) of Hepatitis B

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2001
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/18336/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=1119
• Institution: Universitas Gadjah Mada

ABSTRACT Hepatitis B Virus (HBV) infection is an important health problem in the world including Indonesia. Apart from causing acute hepatitis, HBV infection can also cause chronic liver diseases such as chronic hepatitis, liver cirrhosis and primary liver cancer. The most effective efforts to prevent HBV infection is vaccination. Conventional hepatitis B vaccine used HBsAg derived from carrier plasma. Because of limited number of HBsAg positive blood unit and the concern about the safety of plasma derived vaccine, recombi-nant HBsAg produced by genetics engineering is seriously needed. In some developed countries, recombinant HBsAg has been produced, but in Indonesia, the production of recombinant HBsAg by exploring HBV genome of local isolate has never been reported. The purpose of this research is to isolate and amplify the S gene encoding Hepatitis B Surface Antigen (HBsAg) of local isolates. The result of the amplification was then cloned and expressed in E. coli. The recombinant HBsAg as the product of the expressed S gene in E. coli was then analyzed by immunoscreening, using SDS-PAGE and Western Blot with spesific monoclonal antibody (anti-HBs). The recombinant HBsAg produced was apparently a combination of S protein and pre-S protein with molecular weight of+36 kDa, similar to middle protein HBsAg produced by HBV. The product was neutralized by anti-HBs, and was immunogenic. This result was highly beneficial, because HBsAg which consisted of S protein and pre-S protein was theoretically more immunogenic compared to HBsAg which consisted of S protein only. Therefore, further researches should be conducted to obtain a better recombinant HBsAg, using a more appropriate strategy, among others by using yeast or mammal cells statement system. Keywords: Cloning, S gene � Recombinant, HBsAg � E. coli