IIsolation of GAG-CA Subunit of Jembrana Virus from the Viral Genome by RT-PCR and its cloning in pCR2.1-topolasmid using topoisomerase-based system
Jembrana virus is agent of acute Jembrana virus disease (JVD) in Bos javanicus. The genome of Jembrana virus is composed of a single-stranded RNA. We described in this paper the isolation of gag-ca subunit gene from the viral genome by a single-step RT-PCR reaction. The isolated gene was inserted in...
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Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2002
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Online Access: | https://repository.ugm.ac.id/18409/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=1195 |
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Institution: | Universitas Gadjah Mada |