The effect of horse milk lactoferrin on mice cellular immune response:

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2004
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/18561/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=1353
• Institution: Universitas Gadjah Mada

Lactoferrin (Lf) is an iron-binding glycoprotein, has been known as a nonspecific immunopotentiator. The aims of this research were to study the effect of horse milk lactoferrin on mice lymphocyte proliferation and interleukin (IL-2, IL-4) production. Female Balb/c mice 6-8 weeks old, fed horse milk lactoferrin 1.0 mg/mice/ day, in assay period of 28 day. On the 7th, 14th, 21" and 28th day, lymphocyte proliferation was assayed with MTT, IL-2 and IL-4 were analyzed by ELISA. Result indicate, lymphocyte proliferation of mice fed horse milk lactoferrin was higher than the control and bovine milk lactoferrin on the day of 14th, whereas lymphocyte proliferation on the 28th day of mice fed bovine milk lactoferrin was higher than horse milk lactoferrin and the control. Level of IL-2 and IL-4 in lymphocyte culture, and IL-4 in serum of mice fed horse milk lactoferrin, were higher than the control, whereas serum IL-2 was undetectable. Keywords: Horse milk lactoferrin, lymphocyte proliferation, interleukin production